Methyltransferase-like protein 16 (METTL16) is a human RNA methyltransferase that installs
m6A marks on U6 small nuclear RNA (U6 snRNA) and S-adenosylmethionine (SAM) …

Removal of introns from precursor messenger RNA (pre-mRNA) and some noncoding
transcripts is an essential step in eukaryotic gene expression. In the nucleus, this process of …

Abstract N 6‐methyladenosine (m6A) is a highly dynamic RNA modification that has recently
emerged as a key regulator of gene expression. While many m6A modifications are installed …

U1 snRNP binds to the 5′ exon-intron junction of pre-mRNA and thus plays a crucial role at
an early stage of pre-mRNA splicing. We present two crystal structures of engineered U1 …

Exactly how specific splice sites are recognized during the processing of complex precursor
messenger RNAs is not clear. Small nuclear ribonucleoprotein particles (snRNPs) are …

Five small nuclear RNAs (U1, U2, U4, U5, and U6) participate in precursor messenger RNA
(pre-mRNA) splicing. To probe their interactions within the active center of the mammalian …

Prior to the chemical steps of mRNA splicing, the extensive base-pairing interaction between
the U4 and U6 spliceosomal snRNAs is disrupted. Here, we use a mutational analysis in …

During spliceosome activation, a large structural rearrangement occurs that involves the
release of two small nuclear RNAs, U1 and U4, and the addition of a protein complex …

Using mouse immunoglobulin mu (IgM) pre-mRNA as the model substrate for in vitro
splicing, we have explored the role of exon sequences in splicing. We have found that …

Precursor messenger RNA splicing requires multiple factors including U1, U2, U4, U5, and
U6 small nuclear RNA′ s. The crosslinking reagent psoralen was used to analyze the …