Virulence-associated bacterial type III secretion systems are multiprotein molecular
machines that promote the pathogenicity of bacteria towards eukaryotic host cells. These …

Microbial communities that colonize the human gastrointestinal (GI) tract defend against
pathogens through a mechanism known as colonization resistance (CR). Advances in …

Background The inability of biologics to pass the plasma membrane prevents their
development as therapeutics for intracellular targets. To address the lack of methods for …

New protein-fragment complementation assays (PCA) have successfully been developed to
characterize protein–protein interactions in vitro and in vivo. Notably, the NanoBiT …

The main protease, Mpro, is critical for SARS-CoV-2 replication and an appealing target for
designing anti-SARS-CoV-2 agents. Therefore, there is a demand for the development of …

The independent naming of components of injectisome-type type III secretion systems in
different bacterial species has resulted in considerable confusion, impeding accessibility of …

The plasmids from the Université d'Ottawa (pUdOs) are 28 small plasmids each comprising
one of four origins of replication and one of seven selection markers, which together afford …

Biotherapeutic strategies to promote health, including the application of engineered
microbes to deliver therapeutic molecules, hold strong promise. However, without precision …

Type IV secretion of effector proteins is an important principle for interaction of human
pathogens with their target cells. The corresponding secretion systems may transport a …

The evolutionary conserved YopJ family comprises numerous type-III-secretion system
(T3SS) effectors of diverse mammalian and plant pathogens that acetylate host proteins to …