The CRISPR–Cas toolbox for analytical and diagnostic assay development
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
CRISPR-Cas guides the future of genetic engineering
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
The CRISPR tool kit for genome editing and beyond
M Adli - Nature communications, 2018 - nature.com
CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
Mechanisms regulating zygotic genome activation
KN Schulz, MM Harrison - Nature Reviews Genetics, 2019 - nature.com
Following fertilization, the two specified gametes must unite to create an entirely new
organism. The genome is initially transcriptionally quiescent, allowing the zygote to be …
organism. The genome is initially transcriptionally quiescent, allowing the zygote to be …
CRISPR–Act3. 0 for highly efficient multiplexed gene activation in plants
RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …
However, the simultaneous activation of multiple genes remains challenging. Here, we …
Current and perspective diagnostic techniques for COVID-19
X Yuan, C Yang, Q He, J Chen, D Yu, J Li… - ACS infectious …, 2020 - ACS Publications
Since late December 2019, the coronavirus pandemic (COVID-19; previously known as
2019-nCoV) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …
2019-nCoV) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …
[HTML][HTML] A CRISPR–dCas toolbox for genetic engineering and synthetic biology
Programmable control of gene expression is essential to understanding gene function,
engineering cellular behaviors, and developing therapeutics. Beyond the gene editing …
engineering cellular behaviors, and developing therapeutics. Beyond the gene editing …
CRISPR-mediated live imaging of genome editing and transcription
We report a robust, versatile approach called CRISPR live-cell fluorescent in situ
hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad …
hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad …
Rapid and fully microfluidic Ebola virus detection with CRISPR-Cas13a
Highly infectious illness caused by pathogens is endemic especially in developing nations
where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care …
where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care …