Highlights•Glycosidases carry out many essential functions across all domains of
life.•Activity-based probes can interrogate complex biological samples for glycosidase …

Activity-based protein profiling (ABPP) is an effective technology for the identification and
functional annotation of enzymes in complex biological samples. ABP designs are normally …

α-Glucosidase inhibitors are potential therapeutics for the treatment of diabetes, viral
infections, and Pompe disease. Herein, we report a 1, 6-epi-cyclophellitol cyclosulfamidate …

Retaining glycoside hydrolases use acid/base catalysis with an enzymatic acid/base
protonating the glycosidic bond oxygen to facilitate leaving-group departure alongside …

The crystal structure of the Thermoanaerobacterium xylanolyticum in glycoside hydrolase
family 116 (TxGH116) β-glucosidase provides a structural model for human GBA2 …

Identifying and characterizing the enzymes responsible for an observed activity within a
complex eukaryotic catabolic system remains one of the most significant challenges in the …

Golgi mannosidase II (GMII) catalyzes the sequential hydrolysis of two mannosyl residues
from GlcNAcMan5GlcNAc2 to produce GlcNAcMan3GlcNAc2, the precursor for all complex …

Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels
and other commodities. However, the extraordinary recalcitrance of plant polysaccharides …

Amylases are key enzymes in the processing of starch in many kingdoms of life. They are
important catalysts in industrial biotechnology where they are applied in, among others, food …

The combined inhibition of endoplasmic reticulum (ER) α-glucosidases I and II has been
shown to inhibit replication of a broad range of viruses that rely on ER protein quality control …