Integrative approaches in structural biology: a more complete picture from the combination of individual techniques

L Cerofolini, M Fragai, E Ravera, CA Diebolder… - Biomolecules, 2019 - mdpi.com
With the recent technological and computational advancements, structural biology has
begun to tackle more and more difficult questions, including complex biochemical pathways …

Solid-state NMR methods for the characterization of bioconjugations and protein-material interactions

L Cerofolini, G Parigi, E Ravera, M Fragai… - Solid State Nuclear …, 2022 - Elsevier
Protein solid-state NMR has evolved dramatically over the last two decades, with the
development of new hardware and sample preparation methodologies. This technique is …

Ferritin nanocomposites for the selective delivery of photosensitizing ruthenium-polypyridyl compounds to cancer cells

L Conti, S Ciambellotti, GE Giacomazzo… - Inorganic Chemistry …, 2022 - pubs.rsc.org
Herein, we report on the successful encapsulation into human H-ferritin of two highly
charged Ru (II) polypyridyl complexes,[Ru (phen) 2L′] 2+(Ru1, with L′= 4, 4′-(2, 5, 8, 11 …

Chemistry at the protein–mineral interface in L-ferritin assists the assembly of a functional (μ3-oxo)Tris[(μ2-peroxo)] triiron(III) cluster

C Pozzi, S Ciambellotti… - Proceedings of the …, 2017 - National Acad Sciences
X-ray structures of homopolymeric L-ferritin obtained by freezing protein crystals at
increasing exposure times to a ferrous solution showed the progressive formation of a triiron …

Efficient H2O2 photocatalysis by a novel organic semiconductor with electron donor-acceptor interface

R Ji, Y Dong, X Sun, C Pan, Y Yang, H Zhao… - Applied Catalysis B …, 2024 - Elsevier
Nowadays, considerable attention has been directed towards the two-electron oxygen
reduction for the photocatalytic H 2 O 2 production. However, the challenge of insufficient …

[HTML][HTML] L-Ferritin targets breast cancer stem cells and delivers therapeutic and imaging agents

L Conti, S Lanzardo, R Ruiu, M Cadenazzi, F Cavallo… - Oncotarget, 2016 - ncbi.nlm.nih.gov
A growing body of evidence suggests that cancer stem cells (CSC) have the unique
biological properties necessary for tumor maintenance and spreading, and function as a …

Sedimentation yields long-term stable protein samples as shown by solid-state NMR

T Wiegand, D Lacabanne, A Torosyan… - Frontiers in molecular …, 2020 - frontiersin.org
Today, the sedimentation of proteins into a magic-angle spinning (MAS) rotor gives access
to fast and reliable sample preparation for solid-state Nuclear Magnetic Resonance (NMR) …

Epitope mapping and binding assessment by solid-state NMR provide a way for the development of biologics under the quality by design paradigm

D Rizzo, L Cerofolini, S Giuntini, L Iozzino… - Journal of the …, 2022 - ACS Publications
Multispecific biologics are an emerging class of drugs, in which antibodies and/or proteins
designed to bind pharmacological targets are covalently linked or expressed as fusion …

[HTML][HTML] 19F: A small probe for a giant protein

L Cosottini, S Zineddu, L Massai, V Ghini… - Journal of Inorganic …, 2023 - Elsevier
Herein we describe a method for the efficient production (∼ 90% fluorination) of 5-F-Trp
human H ferritin via the selective incorporation of 19 F into the side chain of W93 using 5 …

Characterization of PEGylated Asparaginase: new opportunities from NMR analysis of large PEGylated therapeutics

L Cerofolini, S Giuntini, A Carlon… - … A European Journal, 2019 - Wiley Online Library
Resonance assignment and structural characterization of pharmacologically relevant
proteins promise to improve understanding and safety of these proteins by rational design …