Molecular detection of antimicrobial resistance

AC Fluit, MR Visser, FJ Schmitz - Clinical microbiology reviews, 2001 - Am Soc Microbiol
The determination of antimicrobial susceptibility of a clinical isolate, especially with
increasing resistance, is often crucial for the optimal antimicrobial therapy of infected …

Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA)

DFJ Brown, DI Edwards, PM Hawkey… - Journal of …, 2005 - academic.oup.com
These evidence-based guidelines have been produced after a literature review of the
laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus …

DNA-based hybridization chain reaction for amplified bioelectronic signal and ultrasensitive detection of proteins

B Zhang, B Liu, D Tang, R Niessner, G Chen… - Analytical …, 2012 - ACS Publications
This work reports a novel electrochemical immunoassay protocol with signal amplification
for determination of proteins (human IgG here used as a model target analyte) at an ultralow …

Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay

P Francois, D Pittet, M Bento, B Pepey… - Journal of Clinical …, 2003 - Am Soc Microbiol
ABSTRACT A rapid procedure was developed for detection and identification of methicillin-
resistant Staphylococcus aureus (MRSA) directly from sterile sites or mixed flora samples …

Rapid Identification of Methicillin-ResistantStaphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR

U Reischl, HJ Linde, M Metz… - Journal of clinical …, 2000 - Am Soc Microbiol
ABSTRACT A duplex LightCycler PCR assay targeting the mecA gene and a
Staphylococcus aureus-specific marker was used to test 165 S. aureus strains and 80 …

Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs

D Jonas, M Speck, FD Daschner… - Journal of Clinical …, 2002 - Am Soc Microbiol
ABSTRACT A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA),
obviating the need for subculture on agar media, was investigated. The combination of …

Multiplex PCR for Simultaneous Identification ofStaphylococcus aureus and Detection of Methicillin and Mupirocin Resistance

E Perez-Roth, F Claverie-Martın, J Villar… - Journal of clinical …, 2001 - Am Soc Microbiol
In this work, we describe a multiplex PCR assay for the detection of clinically relevant
antibiotic resistance genes harbored by some Staphylococcus aureus isolates and for the …

Evaluation of Three Rapid Methods for Detection of Methicillin Resistance in Staphylococcus aureus

L Louie, SO Matsumura, E Choi, M Louie… - Journal of clinical …, 2000 - Am Soc Microbiol
ABSTRACT The probe-based Velogene Rapid MRSA Identification Assay (ID Biomedical
Corp., Vancouver, British Columbia, Canada) and the latex agglutination MRSA-Screen …

PCR in diagnosis of infection: detection of bacteria in cerebrospinal fluids

Y Yamamoto - Clinical and Vaccine Immunology, 2002 - Am Soc Microbiol
The PCR is the most sensitive of the existing rapid methods to detect microbial pathogens in
clinical specimens. In particular, when specific pathogens that are difficult to culture in vitro …

Evaluation of a Triplex PCR Assay To Discriminate Staphylococcus aureus from Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood …

N Maes, J Magdalena, S Rottiers… - Journal of clinical …, 2002 - Am Soc Microbiol
ABSTRACT A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for
identification of staphylococci and detection of methicillin resistance. After validation of the …