Single-cell droplet microfluidics for biomedical applications

D Liu, M Sun, J Zhang, R Hu, W Fu, T Xuanyuan, W Liu - Analyst, 2022 - pubs.rsc.org
Single-cell manipulation and analysis is critical to the study of many fundamental biological
processes and uncovering cellular heterogeneity, and presents the potential for extremely …

Recent advances in single cell manipulation and biochemical analysis on microfluidics

D Gao, F Jin, M Zhou, Y Jiang - Analyst, 2019 - pubs.rsc.org
Single cell analysis has become of great interest with unprecedented capabilities for the
systematic investigation of cell-to-cell variation in large populations. Rapid and multi …

Enhanced single-cell encapsulation in microfluidic devices: From droplet generation to single-cell analysis

SD Ling, Y Geng, A Chen, Y Du, J Xu - Biomicrofluidics, 2020 - pubs.aip.org
Single-cell analysis to investigate cellular heterogeneity and cell-to-cell interactions is a
crucial compartment to answer key questions in important biological mechanisms. Droplet …

A microfluidic device enabling deterministic single cell trapping and release

H Chai, Y Feng, F Liang, W Wang - Lab on a Chip, 2021 - pubs.rsc.org
Successful single-cell isolation is a pivotal technique for subsequent biological and
chemical analysis of single cells. Although significant advances have been made in single …

Particle manipulation methods in droplet microfluidics

M Tenje, A Fornell, M Ohlin, J Nilsson - 2018 - ACS Publications
This Feature describes the different particle manipulation techniques available in the droplet
microfluidics toolbox to handle particles encapsulated inside droplets and to manipulate …

Single-cell patterning: a new frontier in bioengineering

R Gayathri, S Kar, M Nagai, FG Tseng… - Materials Today …, 2022 - Elsevier
Single-cell patterning technologies are rapidly evolving, where single cells are positioned in
spatially distinct areas, thus providing a platform for several biological and biomedical …

An Automated Single‐Cell Droplet‐Digital Microfluidic Platform for Monoclonal Antibody Discovery

F Ahmadi, H Tran, N Letourneau, SR Little, A Fortin… - Small, 2024 - Wiley Online Library
Monoclonal antibody (mAb) discovery plays a prominent role in diagnostic and therapeutic
applications. Droplet microfluidics has become a standard technology for high‐throughput …

One cell, one drop, one click: hybrid microfluidics for mammalian single cell isolation

K Samlali, F Ahmadi, ABV Quach, G Soffer, SCC Shih - Small, 2020 - Wiley Online Library
Generating a stable knockout cell line is a complex process that can take several months to
complete. In this work, a microfluidic method that is capable of isolating single cells in …

A microfluidic array enabling generation of identical biochemical stimulating signals to trapped biological cells for single-cell dynamics

M Yu, YJ Li, YN Yang, CD Xue, GY Xin, B Liu, KR Qin - Talanta, 2024 - Elsevier
Microfluidic-based analyses of single-cell dynamics in response to dynamic biochemical
signals are emerging as pivotal approaches for investigating the effects of extracellular …

Characterisation of anticancer peptides at the single-cell level

L Armbrecht, G Gabernet, F Kurth, JA Hiss… - Lab on a Chip, 2017 - pubs.rsc.org
The development of efficacious anticancer therapeutics is difficult due to the heterogeneity of
the cellular response to chemotherapy. Anticancer peptides (ACPs) are promising drug …