Identification of the High Mannose N-Glycan Isomers Undescribed by Conventional Multicellular Eukaryotic Biosynthetic Pathways

CY Liew, HS Luo, TY Yang, AT Hung… - Analytical …, 2023 - ACS Publications
N-linked glycosylation is one of the most important post-translational modifications of
proteins. Current knowledge of multicellular eukaryote N-glycan biosynthesis suggests high …

Unusual free oligosaccharides in human bovine and caprine milk

WC Weng, HE Liao, SP Huang, ST Tsai, HC Hsu… - Scientific Reports, 2022 - nature.com
Free oligosaccharides are abundant macronutrients in milk and involved in prebiotic
functions and antiadhesive binding of viruses and pathogenic bacteria to colonocytes …

De novo structural determination of oligosaccharide isomers in glycosphingolipids using logically derived sequence tandem mass spectrometry

CY Liew, CK Chan, SP Huang, YT Cheng, ST Tsai… - Analyst, 2021 - pubs.rsc.org
Despite the importance of carbohydrates in biological systems, structural determination of
carbohydrates remains difficult because of the large number of isomers. In this study, a new …

Unusual free trisaccharides in caprine colostrum discovered by logically derived sequence tandem mass spectrometry

WC Weng, HE Liao, CH Chang, SC Hung, K Du… - Scientific Reports, 2025 - nature.com
Free oligosaccharides in human milk have many biological functions for infant health. The
reducing end of most human milk oligosaccharides is lactose, and caprine milk was reported …

Collision-Induced Dissociation of α-Isomaltose and α-Maltose

HS Nguan, CK Ni - The Journal of Physical Chemistry A, 2022 - ACS Publications
Determination of carbohydrate structures remains a considerable challenge. Collision-
induced dissociation (CID) tandem mass spectroscopy (MS/MS) is widely used for …

Collision-Induced Dissociation of Fucose and Identification of Anomericity

HS Nguan, JL Chen, CK Ni - The Journal of Physical Chemistry A, 2024 - ACS Publications
Structural determination of carbohydrates using mass spectrometry remains challenging,
particularly, the differentiation of anomeric configurations. In this work, we studied the …

Collision-Induced Dissociations of Linear Hexose and Disaccharides with Linear Hexose at the Reducing End

HS Nguan, HC Hsu, WL Li, CY Liew… - The Journal of Physical …, 2024 - ACS Publications
Characterization of carbohydrate structures using mass spectrometry is a challenging task.
Understanding the dissociation mechanisms of carbohydrates in the gas phase is crucial for …

A simple tandem mass spectrometry method for structural identification of pentose oligosaccharides

ST Tsai, HC Hsu, CK Ni - Analyst, 2023 - pubs.rsc.org
Differentiation of stereoisomers that are only dissimilar in the orientation of chemical bonds
in space by mass spectrometry remains challenging. Structural determination of …

[HTML][HTML] Xylanases and high-degree wet milling improve soluble dietary fibre content in liquid oat base

M Mohammadi, S Norlander, M Hedström… - Food Chemistry, 2024 - Elsevier
The growth of plant-based food and drink substitutes has led to increased interest in oat-
based milk substitute as a dairy milk alternative. Conventional liquid oat base (LOB) …

The Good, the Bad, and the Ugly Memories of Carbohydrate Fragments in Collision-Induced Dissociation

CY Liew, HC Hsu, HS Nguan, YC Huang… - Journal of the …, 2022 - ACS Publications
Collision-induced dissociation (CID) tandem mass spectrometry is commonly used for
carbohydrate structural determinations. In the CID tandem mass spectrometry approach …