Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by
phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 …

Proteomic analyses of extinct moa (Dinornithidae;~ 800–1000 years) bone tissue previously
revealed preserved collagens (I, II, and V), as well as several biological post-translational …

A lipoxygenase from Pleurotus sajor‐caju (PsLOX) was cloned, expressed in Escherichia
coli, and purified as a soluble protein with a specific activity of 629 μmol/min/mg for …

Two experiments are described that extend the capabilities of quadrupole ion trap mass
spectrometers operated in the precursor and neutral loss scan mode. The first experiment, a …

Identification and quantitation of related impurities is vital in obtaining corrected purity values
for peptide certified reference materials. The sensitivity and selectivity of high-resolution …

Mass spectrometry imaging (MSI) provides spatially resolved information on the molecular
composition of a wide range of samples. Methods and instrumentation are continually being …

Data independent scan modes, such as the precursor and neutral loss scans, enable the
user to quickly identify compounds containing particular functional groups. However …

Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Katedra analytické chemie
Kandidát: Tomáš Šemlej Školitel: PharmDr. Juraj Lenčo, Ph. D. Název diplomové práce …