Thermophilic helicase-dependent amplification-based CRISPR/Cas12a system: Detection of stx2 in Escherichia coli O157: H7 by controlling primer dimers

U Kim, SY Lee, SW Oh - Analytica Chimica Acta, 2023 - Elsevier
Background s: To overcome the limitation of polymerase chain reaction (PCR), isothermal
amplification methods such as thermophilic helicase-dependent amplification (tHDA) have …

A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix

A Ganguli, J Lim, A Mostafa… - Proceedings of the …, 2022 - National Acad Sciences
Blood stream infections (BSIs) cause high mortality, and their rapid detection remains a
significant diagnostic challenge. Timely and informed administration of antibiotics can …

Ultra-sensitive and label-free detection of Escherichia coli O157: H7 using graphene-based field effect transistor modified with heat-denatured casein

S Wei, Y Dou, T Li - Microchemical Journal, 2023 - Elsevier
Sensitive detection of Escherichia coli O157: H7 (E. coli O157: H7) in suspected water
samples is critical for containing the water-borne outbreak, which may cause acute diarrhea …

A Blood Drying Process for DNA Amplification

J Lim, S Zhou, J Baek, AY Kim, E Valera, J Sweedler… - Small, 2024 - Wiley Online Library
The presence of numerous inhibitors in blood makes their use in nucleic acid amplification
techniques difficult. Current methods for extracting and purifying pathogenic DNA from blood …

A biphasic accelerated strand exchange amplification strategy for culture-independent and rapid detection of Salmonella enterica in food samples

X Zhang, X Wang, B Shi, J Li, Y Fan, Y Li, C Shi… - Analytical …, 2024 - pubs.rsc.org
Salmonella enterica is a common foodborne pathogen that can cause food poisoning in
humans. The organism also infects and causes disease in animals. Rapid and sensitive …