Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D
projection images of a tilted biological sample. It allows densities to be resolved in 3D that …

Cryo-electron tomography is a powerful technique that can faithfully image the native
cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo …

We present a new approach for macromolecular structure determination from multiple
particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram …

Macromolecular crowding has a profound impact on reaction rates and the physical
properties of the cell interior, but the mechanisms that regulate crowding are poorly …

Cellular electron cryotomography offers researchers the ability to observe macromolecules
frozen in action in situ, but a primary challenge with this technique is identifying molecular …

Cryo-focused ion beam milling of frozen-hydrated cells has recently provided
unprecedented insights into the inner space of cells. In combination with cryo-electron …

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining
cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron …

The conserved co-activator complex Mediator enables regulated transcription initiation by
RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) …

Cryogenic electron tomography (cryo-ET) visualizes the 3D spatial distribution of
macromolecules at nanometer resolution inside native cells. However, automated …

The 26 S proteasome is a key player in eukaryotic protein quality control and in the
regulation of numerous cellular processes. Here, we describe quantitative in situ structural …