One of the most exciting developments in the field of biological physics in recent years is the
ability to manipulate single molecules and probe their properties and function. Since its …

Mechanical forces regulate a large variety of cellular functionalities, encompassing eg
motility, differentiation and muscle contractility. To adapt to the dynamic change in …

Single-molecule methods have given experimental access to the mechanical properties of
single protein molecules. So far, access has been limited to mostly one spatial direction of …

Protein immobilization is an essential method for both basic and applied research for
protein, and covalent, site-specific attachment is the most desirable strategy. Classic …

The recent development of chemical and bio-conjugation techniques allows for the
engineering of various protein polymers. However, most of the polymerization process is …

The last 15 years saw a dramatic increase in the number of published papers exposing
research related to the concept of protein disorder and a concomitant increase in the …

3, 4-Dihydroxyphenylalanine (DOPA) is the noncanonical amino acid widely found in
mussel holdfast proteins, which is proposed to be responsible for their strong wet adhesion …

Here we report on a method that extends the study of the mechanical behavior of single
proteins to the low force regime of optical tweezers. This experimental approach relies on …

Single-protein force experiments have relied on a molecular fingerprint based on tethering
multiple single-protein domains in a polyprotein chain. However, correlations between these …

Perturbation of a protein away from its native state by mechanical stress is a physiological
process immanent to many cells. The mechanical stability and conformational diversity of …