Naturally occurring modified ribonucleosides
PJ McCown, A Ruszkowska, CN Kunkler… - Wiley …, 2020 - Wiley Online Library
The chemical identity of RNA molecules beyond the four standard ribonucleosides has
fascinated scientists since pseudouridine was characterized as the “fifth” ribonucleotide in …
fascinated scientists since pseudouridine was characterized as the “fifth” ribonucleotide in …
[HTML][HTML] Challenges and emerging trends in liquid chromatography-based analyses of mRNA pharmaceuticals
S Fekete, C Doneanu, B Addepalli, M Gaye… - … of Pharmaceutical and …, 2023 - Elsevier
Lipid encapsulated messenger RNA (LNP mRNA) has garnered a significant amount of
interest from the pharmaceutical industry and general public alike. This attention has been …
interest from the pharmaceutical industry and general public alike. This attention has been …
Full sequencing of CRISPR/Cas9 single guide RNA (sgRNA) via parallel ribonuclease digestions and hydrophilic interaction liquid chromatography–high-resolution …
CRISPR/Cas9 is a powerful genome editing approach in which a Cas9 enzyme and a single
guide RNA (sgRNA) form a ribonucleoprotein complex effectively targeting site-specific …
guide RNA (sgRNA) form a ribonucleoprotein complex effectively targeting site-specific …
Human RNase 4 improves mRNA sequence characterization by LC–MS/MS
With the rapid growth of synthetic messenger RNA (mRNA)-based therapeutics and
vaccines, the development of analytical tools for characterization of long, complex RNAs has …
vaccines, the development of analytical tools for characterization of long, complex RNAs has …
[HTML][HTML] Ushering in the era of tRNA medicines
T Anastassiadis, C Köhrer - Journal of Biological Chemistry, 2023 - Elsevier
Long viewed as an intermediary in protein translation, there is a growing awareness that
transfer RNAs (tRNAs) are capable of myriad other biological functions linked to human …
transfer RNAs (tRNAs) are capable of myriad other biological functions linked to human …
Detection of ribonucleoside modifications by liquid chromatography coupled with mass spectrometry
A small set of ribonucleoside modifications have been found in different regions of mRNA
including the open reading frame. Accurate detection of these specific modifications is …
including the open reading frame. Accurate detection of these specific modifications is …
Oligonucleotide sequence mapping of large therapeutic mRNAs via parallel ribonuclease digestions and LC-MS/MS
Characterization of mRNA sequences is a critical aspect of mRNA drug development and
regulatory filing. Herein, we developed a novel bottom-up oligonucleotide sequence …
regulatory filing. Herein, we developed a novel bottom-up oligonucleotide sequence …
On-line Sequencing of CRISPR Guide RNAs and Their Impurities via the Use of Immobilized Ribonuclease Cartridges Attached to a 2D/3D-LC–MS System
In this study, for the first time, the automated digestion and sequencing of an RNA molecule
via the use of immobilized RNase cartridges attached to a multidimensional liquid …
via the use of immobilized RNase cartridges attached to a multidimensional liquid …
Improved RNA modification mapping of cellular non-coding RNAs using C-and U-specific RNases
P Thakur, M Estevez, PA Lobue, PA Limbach… - Analyst, 2020 - pubs.rsc.org
Locating ribonucleoside modifications within an RNA sequence requires digestion of the
RNA into oligoribonucleotides of amenable size for subsequent analysis by LC-MS (liquid …
RNA into oligoribonucleotides of amenable size for subsequent analysis by LC-MS (liquid …
tRNA modification profiles and codon-decoding strategies in Methanocaldococcus jannaschii
ABSTRACT tRNAs play a critical role in mRNA decoding, and posttranscriptional
modifications within tRNAs drive decoding efficiency and accuracy. The types and positions …
modifications within tRNAs drive decoding efficiency and accuracy. The types and positions …