Genetic alphabet expansion technology by creating unnatural base pairs

M Kimoto, I Hirao - Chemical Society Reviews, 2020 - pubs.rsc.org
Recent advancements in the creation of artificial extra base pairs (unnatural base pairs,
UBPs) are opening the door to a new research area, xenobiology, and genetic alphabet …

The expanded genetic alphabet

DA Malyshev, FE Romesberg - … Chemie International Edition, 2015 - Wiley Online Library
All biological information, since the last common ancestor of all life on Earth, has been
encoded by a genetic alphabet consisting of only four nucleotides that form two base pairs …

A semi-synthetic organism with an expanded genetic alphabet

DA Malyshev, K Dhami, T Lavergne, T Chen, N Dai… - Nature, 2014 - nature.com
Organisms are defined by the information encoded in their genomes, and since the origin of
life this information has been encoded using a two-base-pair genetic alphabet (A–T and G …

Evolution and metabolic significance of the urea cycle in photosynthetic diatoms

AE Allen, CL Dupont, M Oborník, A Horák… - Nature, 2011 - nature.com
Diatoms dominate the biomass of phytoplankton in nutrient-rich conditions and form the
basis of some of the world's most productive marine food webs,,,. The diatom nuclear …

A semisynthetic organism engineered for the stable expansion of the genetic alphabet

Y Zhang, BM Lamb, AW Feldman… - Proceedings of the …, 2017 - National Acad Sciences
All natural organisms store genetic information in a four-letter, two-base-pair genetic
alphabet. The expansion of the genetic alphabet with two synthetic unnatural nucleotides …

New codons for efficient production of unnatural proteins in a semisynthetic organism

EC Fischer, K Hashimoto, Y Zhang… - Nature Chemical …, 2020 - nature.com
Natural organisms use a four-letter genetic alphabet that makes available 64 triplet codons,
of which 61 are sense codons used to encode proteins with the 20 canonical amino acids …

Genetic code expansion: inception, development, commercialization

M Manandhar, E Chun… - Journal of the American …, 2021 - ACS Publications
Virtually all natural proteins are built from only 20 amino acids, and while this makes
possible all the functions they perform, the ability to encode other amino acids selected for …

Plastid proteome prediction for diatoms and other algae with secondary plastids of the red lineage

A Gruber, G Rocap, PG Kroth, EV Armbrust… - The Plant …, 2015 - Wiley Online Library
The plastids of ecologically and economically important algae from phyla such as
stramenopiles, dinoflagellates and cryptophytes were acquired via a secondary …

Optimization of replication, transcription, and translation in a semi-synthetic organism

AW Feldman, VT Dien, RJ Karadeema… - Journal of the …, 2019 - ACS Publications
Previously, we reported the creation of a semi-synthetic organism (SSO) that stores and
retrieves increased information by virtue of stably maintaining an unnatural base pair (UBP) …

Connecting the plastid: transporters of the plastid envelope and their role in linking plastidial with cytosolic metabolism

APM Weber, N Linka - Annual review of plant biology, 2011 - annualreviews.org
Plastids have a multitude of functions in eukaryotic cells, ranging from photosynthesis to
storage, and a role in essential biosynthetic pathways. All plastids are of either primary or …