Fine-tuning cellular physiology in response to intracellular and environmental cues requires
precise temporal and spatial control of gene expression. High-resolution imaging …

The central dogma teaches us that DNA makes RNA, which in turn makes proteins, the main
building blocks of the cell. But this over simplified linear transmission of information …

Cellular stress leads to reprogramming of mRNA translation and formation of stress granules
(SGs), membraneless organelles consisting of mRNA and RNA-binding proteins. Although …

Biomolecular condensates organize biochemical processes at the subcellular level and can
provide spatiotemporal regulation within a cell. Among these, ribonucleoprotein (RNP) …

Local translation allows spatial control of gene expression. Here, we performed a dual
protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP …

Biological phase transitions form membrane-less organelles that generate distinct cellular
environments. How molecules are partitioned between these compartments and the …

To expand the toolbox of imaging in living cells, we have engineered a single-chain variable
fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting …

Spatial regulation of RNA plays a critical role in gene expression regulation and cellular
function. Understanding spatially resolved RNA dynamics and translation is vital for bringing …

Translation control is critical to regulate protein expression. By directly adjusting protein
levels, cells can quickly respond to dynamic transitions during stem cell differentiation and …

Ribosome profiling, which is based on deep sequencing of ribosome footprints, has served
as a powerful tool for elucidating the regulatory mechanism of protein synthesis. However …