Current strategies for protein production and purification enabling membrane protein structural biology

A Pandey, K Shin, RE Patterson… - Biochemistry and Cell …, 2016 - cdnsciencepub.com
Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …

Engineered Chimera Protein Constructs to Facilitate the Production of Heterologous Transmembrane Proteins in E. coli

A Ogunbowale, ER Georgieva - International Journal of Molecular …, 2024 - mdpi.com
To delve into the structure–function relationship of transmembrane proteins (TMPs), robust
protocols are needed to produce them in a pure, stable, and functional state. Among all …

Hypoxia activates a neuropeptidergic pathway from the paraventricular nucleus of the hypothalamus to the nucleus tractus solitarii

BC Ruyle, PJ Klutho, CP Baines… - American Journal …, 2018 - journals.physiology.org
The paraventricular nucleus of the hypothalamus (PVN) contributes to both autonomic and
neuroendocrine function. PVN lesion or inhibition blunts cardiorespiratory responses to …

Design of an effective small expression tag to enhance GPCR production in E. coli‐based cell‐free and whole cell expression systems

S Cho, H Lee, YH Han, TS Park, SW Seo… - Protein …, 2023 - Wiley Online Library
G protein‐coupled receptors (GPCRs) play crucial roles in sensory, immune, and tumor
metastasis processes, making them valuable targets for pharmacological and sensing …

[HTML][HTML] The Cytoplasmic and periplasmic expression levels and folding of organophosphorus hydrolase enzyme in Escherichia coli

AM Latifi, K Khajeh, G Farnoosh… - Jundishapur journal …, 2015 - ncbi.nlm.nih.gov
Background: Organophosphorus hydrolase (OPH) is a type of organophosphate-degrading
enzyme which is widely used in the bioremediation process. Objectives: In this study, the …

[HTML][HTML] Corticotropin-releasing factor family peptide signaling in feline bladder urothelial cells

AT Hanna-Mitchell, A Wolf-Johnston… - The Journal of …, 2014 - ncbi.nlm.nih.gov
Corticotropin-releasing (CRF) factor plays a central role in the orchestration of behavioral
and neuroendocrine responses to stress. The family of CRF-related peptides (CRF and …

Role of corticotropin-releasing factor on bladder function in rats with psychological stress

M Seki, XM Zha, S Inamura, M Taga, Y Matsuta… - Scientific Reports, 2019 - nature.com
Stress-related peptide corticotropin-releasing factor (CRF) and CRF-related peptides are
distributed in the peripheral viscera such as the bladder. We investigated the contribution of …

Buserelin treatment to rats causes enteric neurodegeneration with moderate effects on CRF-immunoreactive neurons and Enterobacteriaceae in colon, and in …

E Sand, C Linninge, L Lozinska, E Egecioglu, B Roth… - BMC research …, 2015 - Springer
Background The gonadotropin-releasing hormone (GnRH) analog buserelin causes enteric
neuronal loss. Acute stress or injection of corticotropin-releasing factor (CRF) affects motility …

Congenital vasopressin deficiency and acute and chronic opiate effects on hypothalamo-pituitary–adrenal axis activity in Brattleboro rats

A Domokos, Z Mergl, I Barna… - Journal of …, 2007 - joe.bioscientifica.com
There are sex differences in the response to stress and in the influence of stress on
reproduction which may be due to gonadal steroids but the nature of these differences and …

[PDF][PDF] Prokaryotic expression of bioactive zinc transporter 8 antigens and detection of diabetes specific autoantibodies in a single dot immunogold filtration assay

L Cheng, T Li, D Zhang, B Chen - Clin Lab, 2015 - researchgate.net
Background: Type 1 diabetes mellitus is an autoimmune disease, and islet autoantibodies
secreted by auto-reactive plasma cells are diagnostic indicators of the immune processes …