Surveying membrane landscapes: a new look at the bacterial cell surface

T Lithgow, CJ Stubenrauch, MPH Stumpf - Nature Reviews Microbiology, 2023 - nature.com
Recent studies applying advanced imaging techniques are changing the way we
understand bacterial cell surfaces, bringing new knowledge on everything from single-cell …

Targeting BAM for novel therapeutics against pathogenic gram-negative bacteria

C Overly Cottom, R Stephenson, L Wilson, N Noinaj - Antibiotics, 2023 - mdpi.com
The growing emergence of multidrug resistance in bacterial pathogens is an immediate
threat to human health worldwide. Unfortunately, there has not been a matching increase in …

Structural basis of BAM-mediated outer membrane β-barrel protein assembly

C Shen, S Chang, Q Luo, KC Chan, Z Zhang, B Luo… - Nature, 2023 - nature.com
The outer membrane structure is common in Gram-negative bacteria, mitochondria and
chloroplasts, and contains outer membrane β-barrel proteins (OMPs) that are essential …

Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding

MT Doyle, JR Jimah, T Dowdy, SI Ohlemacher… - Cell, 2022 - cell.com
Transmembrane β barrel proteins are folded into the outer membrane (OM) of Gram-
negative bacteria by the β barrel assembly machinery (BAM) via a poorly understood …

A multipoint guidance mechanism for β-barrel folding on the SAM complex

H Takeda, JV Busto, C Lindau, A Tsutsumi… - Nature structural & …, 2023 - nature.com
Mitochondrial β-barrel proteins are essential for the transport of metabolites, ions and
proteins. The sorting and assembly machinery (SAM) mediates their folding and membrane …

Darobactin B Stabilises a Lateral‐Closed Conformation of the BAM Complex in E. coli Cells

SF Haysom, J Machin, JM Whitehouse… - Angewandte Chemie …, 2023 - Wiley Online Library
The β‐barrel assembly machinery (BAM complex) is essential for outer membrane protein
(OMP) folding in Gram‐negative bacteria, and represents a promising antimicrobial target …

Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding

B Schiffrin, JM Machin, TK Karamanos… - Communications …, 2022 - nature.com
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-
negative bacteria depends on delivery of unfolded OMPs to the β-barrel assembly …

Drug binding to BamA targets its lateral gate

KM Kuo, J Liu, A Pavlova… - The Journal of Physical …, 2023 - ACS Publications
BamA, the core component of the β-barrel assembly machinery (BAM) complex, is an outer-
membrane protein (OMP) in Gram-negative bacteria. Its function is to insert and fold …

BamE directly interacts with BamA and BamD coordinating their functions

S Kumar, A Konovalova - Molecular Microbiology, 2023 - Wiley Online Library
The β‐barrel assembly machinery (Bam) complex facilitates the assembly of outer
membrane proteins (OMPs) in gram‐negative bacteria. The Bam complex is conserved and …

Cracking outer membrane biogenesis

RL Guest, TJ Silhavy - Biochimica et Biophysica Acta (BBA)-Molecular Cell …, 2023 - Elsevier
The outer membrane is a distinguishing feature of the Gram-negative envelope. It lies on the
external face of the peptidoglycan sacculus and forms a robust permeability barrier that …