The use of fossil fuels has been strongly related to critical problems currently affecting
society, such as: global warming, global greenhouse effects and pollution. These problems …

Industrial strain development requires system-wide engineering and optimization of cellular
metabolism while considering industrially relevant fermentation and recovery processes. It …

Microalgae are the widely employed sources of third generation biofuels, which attracted
huge attention by the researchers of the present decade because of their great productivity …

Efficient genome engineering is critical to understand and use microbial functions. Despite
recent development of tools such as CRISPR-Cas gene editing, efficient integration of …

Microalgae serve as a promising source for the production of biofuels and bio‐based
chemicals. They are superior to terrestrial plants as feedstock in many aspects and their …

Non-model microorganisms often possess complex phenotypes that could be important for
the future of biofuel and chemical production. They have received significant interest the last …

In this study, CRISPRi (clustered regularly interspaced short palindromic repeats
interference) was used for the first time to regulate expression of exogenously supplied rfp …

Extremophiles are suitable chassis for developing the next generation industrial
biotechnology (NGIB) due to their resistance to microbial contamination. However …

Genome engineering is crucial for answering fundamental questions about, and exploring
practical applications of, microorganisms. Various microbial genome-engineering tools …

Metabolic engineering often necessitates chromosomal integration of multiple genes but
integration of large genes into Escherichia coli remains difficult. CRISPR/Cas9 is an RNA …