Polymerase chain reaction (PCR) is a molecular biology technique used to multiply certain
deoxyribonucleic acid (DNA) fragments. It is a common and indispensable technique that …

In this review we examine the literature related to emerging technologies that will help to
reshape the clinical microbiology laboratory. These topics include nucleic acid amplification …

Clinical microbiology laboratories rely on quantitative PCR for its speed, sensitivity,
specificity and ease-of-use. However, quantitative PCR quantitation requires the use of a …

Pathogens pose a severe threat to food safety and human health. The traditional methods
for pathogen detection can't meet the growing diagnosis and control need. Digital PCR …

BACKGROUND The PCR and its variant, quantitative PCR (qPCR), have revolutionized the
practice of clinical microbiology. Continued advancements in PCR have led to a new …

Two years ago, we described the first droplet digital PCR (ddPCR) system aimed at
empowering all researchers with a tool that removes the substantial uncertainties associated …

Here we report on the first assessment of droplet digital PCR (ddPCR) for detection and
absolute quantification of two quarantine plant pathogenic bacteria that infect many species …

The incidence of opportunistic yeast infections in humans has been increasing over recent
years. These infections are difficult to treat and diagnose, in part due to the large number …

Success in synthetic biology and metabolic engineering is quickly becoming 'test'limited
within the design–build–test cycle. Commonly used methods for high-throughput screening …

Bacterial strain YC-RL4, capable of utilizing phthalic acid esters (PAEs) as the sole carbon
source for growth, was isolated from petroleum-contaminated soil. Strain YC-RL4 was …