Enhancing aquaculture disease resistance: Antimicrobial peptides and gene editing

J Wang, Y Cheng - Reviews in Aquaculture, 2024 - Wiley Online Library
Previous studies have demonstrated that CRISPR/Cas9‐mediated genome editing and
transgenesis by integrating vector‐engineered antimicrobial peptide genes (AMGs) are …

Harnessing CRISPR/Cas9 system to improve economic traits in aquaculture species

A Mokrani, S Liu - Aquaculture, 2024 - Elsevier
Clustered regularly interspaced short palindromic repeats associated with Cas9
(CRISPR/Cas9) system is a powerful and efficient tool for molecular modification in the …

One-step knock-in of two antimicrobial peptide transgenes at multiple loci of catfish by CRISPR/Cas9-mediated multiplex genome engineering

J Wang, IM Torres, M Shang, J Al-Armanazi… - International Journal of …, 2024 - Elsevier
CRISPR/Cas9-mediated multiplex genome e diting (MGE) conventionally uses multiple
single-guide RNAs (sgRNAs) for gene-targeted mutagenesis via the non-homologous end …

Integration of alligator cathelicidin gene via two CRISPR/Cas9-assisted systems enhances bacterial resistance in blue catfish, Ictalurus furcatus

J Wang, B Su, J Al-Armanazi, AL Wise, M Shang… - Aquaculture, 2023 - Elsevier
CRISPR/Cas9-mediated genome editing has paved new avenues for improving production-
valued traits in aquaculture by knocking out or disrupting functional genes. However …

CRISPR/Cas9 microinjection of transgenic embryos enhances the dual-gene integration efficiency of antimicrobial peptide genes for bacterial resistance in channel …

J Wang, B Su, TJ Bruce, AL Wise, P Zeng, G Cao… - Aquaculture, 2023 - Elsevier
Integrating a vector-engineered antimicrobial peptide gene (AMG) into the fish genome
effectively modulated the innate immune system and increased resistance to infectious …

Genome Manipulation Advances in Selected Aquaculture Organisms

J Wang, Y Cheng, B Su, RA Dunham - Reviews in Aquaculture, 2025 - Wiley Online Library
With the rising global demand for seafood and the challenges posed by overfishing and
climate change, the aquaculture sector has become increasingly important in providing high …

CRISPR/Cas9-mediated precision integration of fat-1 and fat-2 from Caenorhabditis elegans at long repeated sequence in channel catfish (Ictalurus punctatus) and …

D Xing, M Shang, S Li, W Wang, T Hasin… - Aquaculture, 2023 - Elsevier
Omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially eicosapentaenoic acid (EPA)
and docosahexaenoic acid (DHA), are essential for the development and health of human …

[HTML][HTML] Genes for editing to improve economic traits in aquaculture fish species

Z Yang, G Fu, M Lee, S Yeo, GH Yue - Aquaculture and Fisheries, 2024 - Elsevier
Aquaculture, a critical sector for global food security, faces the challenge of meeting growing
demand while protecting wild fish populations. Gene editing, a powerful genetic tool …

New Genomic Techniques (NGT) in animals and their agri/food/feed products

AL Van Eenennaam - EFSA Supporting Publications, 2023 - Wiley Online Library
This report presents a review of the commercial and pre‐commercial stage applications of
new genomic technologies (NGT) applied to farm animals and their agri/food/feed products …

[HTML][HTML] Generation of Eco-Friendly and Disease-Resistant Channel Catfish (Ictalurus punctatus) Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 …

J Wang, B Su, D Xing, TJ Bruce, S Li, L Bern, M Shang… - Engineering, 2024 - Elsevier
As a precise and versatile tool for genome manipulation, the clustered regularly interspaced
short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) platform holds …