The precise modification or functionalization of the protein C-terminus is essential but full of
challenges. Herein, a chemical approach to modify the C-terminus is developed by fusing a …

Native chemical ligation (NCL) has been playing an increasingly important role in chemical
protein synthesis (CPS). More efficient ligation methods that circumvent the requirement of a …

Peptide asparaginyl ligases (PALs) hold significant potential in protein bioconjugation due
to their excellent kinetic properties and broad substrate compatibility. However, realizing …

In recent years, a few asparaginyl endopeptidases (AEPs) from certain higher plants have
been identified as efficient peptide ligases with wide applications in protein labeling and …

We describe a practical strategy for ligation of peptide hydrazide at Gln and Asn sites.
Peptide hydrazides with Gln, Asn and Asp at the C-terminus would form by-products during …

The immobilization of proteins on nanoparticles has received much attention in recent years.
Among different approaches, enzymatic protein immobilization shows unique advantages …

A variant originated from Oldenlandia affinis asparaginyl ligase, OaAEP1-C247A, has
emerged as an ideal tool for protein labeling. However, its preparation was laborious and …

Peptidyl Asx-specific ligases (PALs) function as molecular gluing machines to join peptide
and proteins together at Asn or Asp junctions. Because of their highly specific and efficient …