The need for novel cryoprotectants and cryopreservation protocols: Insights into the importance of biophysical investigation and cell permeability

R Raju, SJ Bryant, BL Wilkinson, G Bryant - Biochimica et Biophysica Acta …, 2021 - Elsevier
Background Cryopreservation is a key method of preservation of biological material for both
medical treatments and conservation of endangered species. In order to avoid cellular …

Seed longevity—The evolution of knowledge and a conceptual framework

J Nadarajan, C Walters, HW Pritchard, D Ballesteros… - Plants, 2023 - mdpi.com
The lifespan or longevity of a seed is the time period over which it can remain viable. Seed
longevity is a complex trait and varies greatly between species and even seed lots of the …

The unique role of seed banking and cryobiotechnologies in plant conservation

C Walters, VC Pence - Plants, People, Planet, 2021 - Wiley Online Library
Societal Impact Statement Eroding plant diversity has serious implications for the well‐being
of humanity and our planet. Conserving plants ex situ requires technologies that are rapidly …

Cryopreservation of phytodiversity: a critical appraisal of theory & practice

EE Benson - Critical reviews in Plant sciences, 2008 - Taylor & Francis
Cryopreservation is the storage of viable cells, tissues, organs and organisms at ultra-low
temperatures, usually in liquid nitrogen to a minimum temperature of− 196° C. The term …

Structural mechanics of seed deterioration: standing the test of time

C Walters, D Ballesteros, VA Vertucci - Plant Science, 2010 - Elsevier
Seeds die inevitably but unexpectedly during storage and current understanding of seed
quality and storage conditions do not allow reliable means to predict or prevent this critical …

Vitrification solutions for plant cryopreservation: Modification and properties

J Zamecnik, M Faltus, A Bilavcik - Plants, 2021 - mdpi.com
Many plants cannot vitrify themselves because they lack glassy state-inducing substances
and/or have high water content. Therefore, cryoprotectants are used to induce vitrification. A …

Cryopreservation theory

EE Benson - Plant cryopreservation: a practical guide, 2008 - Springer
The behaviour of water in liquid and frozen states is largely dictated by its bipolar chemistry.
Its hydrogen atoms carry a slight net positive charge and act with unpaired “negatively …

Cryopreservation of agronomic plant germplasm using vitrification-based methods: An overview of selected case studies

CA Roque-Borda, D Kulus, A Vacaro de Souza… - International Journal of …, 2021 - mdpi.com
Numerous environmental and endogenous factors affect the level of genetic diversity in
natural populations. Genetic variability is the cornerstone of evolution and adaptation of …

Frozen beauty: The cryobiotechnology of orchid diversity

E Popova, HH Kim, PK Saxena, F Engelmann… - Biotechnology …, 2016 - Elsevier
Orchids (Orchidaceae) are one of the most diverse plant groups on the planet with over
25,000 species. For over a century, scientists and horticulturalists have been fascinated by …

[HTML][HTML] Innovative approaches to the preservation of forest trees

HW Pritchard, JF Moat, JBS Ferraz, TR Marks… - Forest ecology and …, 2014 - Elsevier
The recent acceleration of actions to conserve plant species using ex situ and in situ
strategies has revealed the need to understand how these two approaches might be better …