The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

For a little more than a century, fluorescence microscopy has been an essential source of
major discoveries in cell biology. Recent developments improved both visualization and …

Because the degree of labeling (DOL) of cell-bound antibodies, often required in
quantitative fluorescence measurements, is largely unknown, we investigated the effect of …

Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are
complementary techniques that can be used to extract information about macromolecules …

Fluorescence-anisotropy-based homo-FRET detection methods can be employed to study
clustering of identical proteins in cells. Here, the potential of fluorescence anisotropy …

Ligand-driven dimerizations of ErbB receptor subunits fulfill a fundamental role in their
activation. We have used the number and brightness analysis technique to investigate the …

Förster resonance energy transfer (FRET), in most applications used as a “spectroscopic
ruler,” allows an easy determination of the donor-acceptor intermolecular distance …

The surface of eukaryotic cells is a multi-component fluid bilayer in which
glycosylphosphatidylinositol (GPI)-anchored proteins are an abundant constituent. In this …

Many important biological functions, and dysfunctions, involve the self-assembly of
molecules. Two well-characterised examples are the clustering of signalling proteins in …