Tracking single molecules at work in living cells

A Kusumi, TA Tsunoyama, KM Hirosawa… - Nature chemical …, 2014 - nature.com
Methods for imaging and tracking single molecules conjugated with fluorescent probes,
called single-molecule tracking (SMT), are now providing researchers with the …

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria

JE Horne, DJ Brockwell, SE Radford - Journal of Biological Chemistry, 2020 - ASBMB
β-Barrel outer membrane proteins (OMPs) represent the major proteinaceous component of
the outer membrane (OM) of Gram-negative bacteria. These proteins perform key roles in …

Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane

TK Fujiwara, K Iwasawa, Z Kalay… - Molecular biology of …, 2016 - Am Soc Cell Biol
The mechanisms by which the diffusion rate in the plasma membrane (PM) is regulated
remain unresolved, despite their importance in spatially regulating the reaction rates in the …

Single-molecule dynamics show a transient lipopolysaccharide transport bridge

L Törk, CB Moffatt, TG Bernhardt, EC Garner, D Kahne - Nature, 2023 - nature.com
Gram-negative bacteria are surrounded by two membranes. A special feature of the outer
membrane is its asymmetry. It contains lipopolysaccharide (LPS) in the outer leaflet and …

Probing the effect of clustering on EphA2 receptor signaling efficiency by subcellular control of ligand-receptor mobility

Z Chen, D Oh, KH Biswas, R Zaidel-Bar, JT Groves - Elife, 2021 - elifesciences.org
Clustering of ligand: receptor complexes on the cell membrane is widely presumed to have
functional consequences for subsequent signal transduction. However, it is experimentally …

Unveiling the inner workings of live bacteria using super-resolution microscopy

HH Tuson, JS Biteen - Analytical chemistry, 2015 - ACS Publications
Optical microscopy has proven to be one of the most powerful tools in biology, in part
because it allows direct, noninvasive visualization of dynamic processes inside live cells. It …

Intermembrane crosstalk drives inner-membrane protein organization in Escherichia coli

P Rassam, KR Long, R Kaminska, DJ Williams… - Nature …, 2018 - nature.com
Gram-negative bacteria depend on energised protein complexes that connect the two
membranes of the cell envelope. However, β-barrel outer-membrane proteins (OMPs) and α …

Actin filaments partition primary cilia membranes into distinct fluid corrals

S Lee, HY Tan, II Geneva, A Kruglov… - Journal of Cell Biology, 2018 - rupress.org
Physical properties of primary cilia membranes in living cells were examined using two
independent, high-spatiotemporal-resolution approaches: fast tracking of single quantum …

ROSET model of TonB action in gram-negative bacterial iron acquisition

PE Klebba - Journal of bacteriology, 2016 - Am Soc Microbiol
The ro tational s urveillance and e nergy t ransfer (ROSET) model of TonB action suggests a
mechanism by which the electrochemical proton gradient across the Gram-negative …

Complex diffusion in bacteria

CH Bohrer, J Xiao - Physical Microbiology, 2020 - Springer
Diffusion within bacteria is often thought of as a “simple” random process by which
molecules collide and interact with each other. New research however shows that this is far …