Viscosity dependence of some protein and enzyme reaction rates: seventy-five years after Kramers

P Sashi, AK Bhuyan - Biochemistry, 2015 - ACS Publications
Kramers rate theory is a milestone in chemical reaction research, but concerns regarding the
basic understanding of condensed phase reaction rates of large molecules in viscous milieu …

Glycerol-slaved 1H-1H NMR cross-relaxation in quasi-native lysozyme

K Joshi, AK Bhuyan - Biophysical Chemistry, 2024 - Elsevier
Abstract 1 H-1 H nuclear cross-relaxation experiments have been carried out with lysozyme
in variable glycerol viscosity to study intramolecular motion, self-diffusion, and isotropic rigid …

Slowed Dynamics of Thin Filament Regulatory Units Reduces Ca2+-Sensitivity of Cardiac Biomechanical Function

CKP Loong, AK Takeda, MA Badr, JS Rogers… - Cellular and molecular …, 2013 - Springer
Actomyosin kinetics in both skinned skeletal muscle fibers at maximum Ca 2+-activation and
unregulated in vitro motility assays are modulated by solvent microviscosity in a manner …

Photoinduced shuttling dynamics of rotaxanes in viscous polymer solutions

DC Jagesar, SM Fazio, J Taybi, E Eiser… - Advanced Functional …, 2009 - Wiley Online Library
The effect of external friction, caused by medium viscosity, on the photoinduced translational
motion in a rotaxane‐based molecular shuttle 1 is investigated. The shuttle is successfully …

Role of macromolecular crowding and salt ions on the structural-fluctuation of a highly compact configuration of carbonmonoxycytochrome c

R Kumar, D Sharma, R Jain, S Kumar - Biophysical chemistry, 2015 - Elsevier
Carbonmonoxycytochrome c refolds to a native-like compact state (NCO-state), where the
non-native Fe 2+-CO interaction persists. Structural and molecular properties extracted from …

Analysis of the pH-dependent thermodynamic stability, local motions, and microsecond folding kinetics of carbonmonoxycytochrome c

R Kumar - Archives of Biochemistry and Biophysics, 2016 - Elsevier
This paper analyzes the effect of pH on thermodynamic stability, low-frequency local motions
and microsecond folding kinetics of carbonmonoxycytochrome c (Cyt-CO) all across the …

Expansion and internal friction in unfolded protein chain

UM Yasin, P Sashi, AK Bhuyan - The Journal of Physical …, 2013 - ACS Publications
Similarities in global properties of homopolymers and unfolded proteins provide approaches
to mechanistic description of protein folding. Here, hydrodynamic properties and relaxation …

Analysis of the effect of 1-Allyl-3-Methylimidazolium chloride on thermodynamic stability, folding kinetics, and motional dynamics of horse cytochrome c

M Garg, D Sharma, R Kumar - Biophysical Chemistry, 2022 - Elsevier
allyl-3-methylimidazolium chloride (AMIMCl) acts as a potential green solvent for proteins.
The present work provides a possible pathway by which the structural, kinetic …

Model for solvent viscosity effect on enzymatic reactions

AE Sitnitsky - Chemical Physics, 2010 - Elsevier
The solvent viscosity dependence for enzymatic reactions is discussed. We suggest the
interpretation of the phenomenon that requires neither a modification of the Kramers' theory …

Viscosity-dependent structural fluctuation of the M80-containing Ω-loop of horse ferrocytochrome c

R Kumar, R Jain - Chemical Physics, 2013 - Elsevier
To determine the effect of solvent viscosity on low-frequency local motions that control the
slow changes in structural dynamics of proteins, we have studied the effects of solvent …