Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy

EE Diel, JW Lichtman, DS Richardson - Nature protocols, 2020 - nature.com
Spherical aberration (SA) occurs when light rays entering at different points of a spherical
lens are not focused to the same point of the optical axis. SA that occurs inside the lens …

Enhancing circulating tumor cell capture: Amin-functionalized bilayer graphene biosensing with integrated chip-level system for point-of-care testing

TL Lee, SC Huang, CC Huang, SW Lee, CH Huang… - Carbon, 2024 - Elsevier
Current research on the detection of circulating tumor cells (CTCs) as a diagnostic strategy
is still in its early stages. Consequently, there is a need for an efficient platform to capture …

Fast and accurate three-dimensional point spread function computation for fluorescence microscopy

J Li, F Xue, T Blu - JOSA A, 2017 - opg.optica.org
The point spread function (PSF) plays a fundamental role in fluorescence microscopy. A
realistic and accurately calculated PSF model can significantly improve the performance in …

[HTML][HTML] Unpaired mesh-to-image translation for 3D fluorescent microscopy images of neurons

M Cudic, JS Diamond, JA Noble - Medical Image Analysis, 2023 - Elsevier
Abstract While Generative Adversarial Networks (GANs) can now reliably produce realistic
images in a multitude of imaging domains, they are ill-equipped to model thin, stochastic …

Towards optimal point spread function design for resolving closely spaced emitters in three dimensions

JM Jusuf, MD Lew - Optics Express, 2022 - opg.optica.org
The past decade has brought many innovations in optical design for 3D super-resolution
imaging of point-like emitters, but these methods often focus on single-emitter localization …

Calculating point spread functions: methods, pitfalls, and solutions

R Holinirina Dina Miora, E Rohwer, M Kielhorn… - Optics …, 2024 - opg.optica.org
The knowledge of the exact structure of the optical system point spread function (PSF)
enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF …

Impact of a tilted coverslip on two-photon and STED microscopy

G Le Bourdellès, L Mercier, J Roos… - Biomedical Optics …, 2024 - opg.optica.org
The advent of super-resolution microscopy has opened up new avenues to unveil brain
structures with unprecedented spatial resolution in the living state. Yet, its application to live …

Modeling point spread function in fluorescence microscopy with a sparse gaussian mixture: Tradeoff between accuracy and efficiency

DK Samuylov, P Purwar, G Székely… - IEEE Transactions on …, 2019 - ieeexplore.ieee.org
Deblurring is a fundamental inverse problem in bioimaging. It requires modeling the point
spread function (PSF), which captures the optical distortions entailed by the image formation …

3D Reconstruction of cellular images from microfabricated imagers using fully-adaptive deep neural networks

H Najafiaghdam, R Rabbani, A Gharia… - Scientific Reports, 2022 - nature.com
Millimeter-scale multi-cellular level imagers enable various applications, ranging from
intraoperative surgical navigation to implantable sensors. However, the tradeoffs for …

[HTML][HTML] Interactive simulation and visualization of point spread functions in single molecule imaging

MC Schneider, F Hinterer, A Jesacher, GJ Schütz - Optics Communications, 2024 - Elsevier
The point spread function (PSF) is fundamental to any type of microscopy, most importantly
so for single-molecule localization techniques, where the exact PSF shape is crucial for …