Long-read sequencing–a powerful tool in viral transcriptome research
Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …
novo assembly and in resolving complex DNA regions as well as in determining full-length …
Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
DP Depledge, KP Srinivas, T Sadaoka… - Nature …, 2019 - nature.com
Characterizing complex viral transcriptomes by conventional RNA sequencing approaches
is complicated by high gene density, overlapping reading frames, and complex splicing …
is complicated by high gene density, overlapping reading frames, and complex splicing …
Alphaherpesvirus genomics: past, present and future
Alphaherpesviruses, as large double-stranded DNA viruses, were long considered to be
genetically stable and to exist in a homogeneous state. Recently, the proliferation of high …
genetically stable and to exist in a homogeneous state. Recently, the proliferation of high …
Novel viral splicing events and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts
Adenovirus is a common human pathogen that relies on host cell processes for transcription
and processing of viral RNA and protein production. Although adenoviral promoters, splice …
and processing of viral RNA and protein production. Although adenoviral promoters, splice …
ATM, KAP1 and the Epstein–Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication
The timing of transcription and replication must be carefully regulated for heavily-transcribed
genomes of double-stranded DNA viruses: transcription of immediate early/early genes must …
genomes of double-stranded DNA viruses: transcription of immediate early/early genes must …
Long-read isoform sequencing reveals a hidden complexity of the transcriptional landscape of herpes simplex virus type 1
In this study, we used the amplified isoform sequencing technique from Pacific Biosciences
to characterize the poly (A)+ fraction of the lytic transcriptome of the herpes simplex virus …
to characterize the poly (A)+ fraction of the lytic transcriptome of the herpes simplex virus …
Identification of herpesvirus transcripts from genomic regions around the replication origins
Long-read sequencing (LRS) techniques enable the identification of full-length RNA
molecules in a single run eliminating the need for additional assembly steps. LRS research …
molecules in a single run eliminating the need for additional assembly steps. LRS research …
Expansion of single cell transcriptomics data of SARS-CoV infection in human bronchial epithelial cells to COVID-19
R Zolfaghari Emameh, H Nosrati, M Eftekhari… - Biological procedures …, 2020 - Springer
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the
causative agent of coronavirus disease 19 (COVID-19) that was emerged as a new member …
causative agent of coronavirus disease 19 (COVID-19) that was emerged as a new member …
Multi-platform sequencing approach reveals a novel transcriptome profile in pseudorabies virus
Third-generation sequencing is an emerging technology that is capable of solving several
problems that earlier approaches were not able to, including the identification of transcripts …
problems that earlier approaches were not able to, including the identification of transcripts …
Third-generation sequencing reveals extensive polycistronism and transcriptional overlapping in a baculovirus
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the …
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the …