Current development of siRNA bioconjugates: from research to the clinic
IV Chernikov, VV Vlassov… - Frontiers in …, 2019 - frontiersin.org
Small interfering RNAs (siRNAs) acting via RNA interference mechanisms are able to
recognize a homologous mRNA sequence in the cell and induce its degradation. The main …
recognize a homologous mRNA sequence in the cell and induce its degradation. The main …
Regulation of small RNA stability: methylation and beyond
As central components of RNA silencing, small RNAs play diverse and important roles in
many biological processes in eukaryotes. Aberrant reduction or elevation in the levels of …
many biological processes in eukaryotes. Aberrant reduction or elevation in the levels of …
Progress toward in vivo use of siRNAs-II
GR Rettig, MA Behlke - Molecular therapy, 2012 - cell.com
RNA interference (RNAi) has been extensively employed for in vivo research since its use
was first demonstrated in mammalian cells 10 years ago. Design rules have improved, and it …
was first demonstrated in mammalian cells 10 years ago. Design rules have improved, and it …
Development of therapeutic-grade small interfering RNAs by chemical engineering
JB Bramsen, J Kjems - Frontiers in genetics, 2012 - frontiersin.org
Recent successes in clinical trials have provided important proof of concept that small
interfering RNAs (siRNAs) indeed constitute a new promising class of therapeutics. Although …
interfering RNAs (siRNAs) indeed constitute a new promising class of therapeutics. Although …
Type 2 diabetes mellitus: limitations of conventional therapies and intervention with nucleic acid-based therapeutics
Figures released in 2013 by the International Diabetes Federation (IDF) state that over 382
million individuals worldwide are affected by diabetes mellitus (DM). 1 The global …
million individuals worldwide are affected by diabetes mellitus (DM). 1 The global …
Antisense oligonucleotides internally labeled with peptides show improved target recognition and stability to enzymatic degradation
Specific target binding and stability in diverse biological media is of crucial importance for
applications of synthetic oligonucleotides as diagnostic and therapeutic tools. So far, these …
applications of synthetic oligonucleotides as diagnostic and therapeutic tools. So far, these …
[HTML][HTML] Development of a highly stable, active small interfering RNA with broad activity against SARS-CoV viruses
B Tolksdorf, J Heinze, D Niemeyer, V Röhrs, J Berg… - Antiviral Research, 2024 - Elsevier
Abstract Treatment options for COVID-19 remain limited. Here, we report the optimization of
an siRNA targeting the highly conserved leader region of SARS-CoV-2. The siRNA was …
an siRNA targeting the highly conserved leader region of SARS-CoV-2. The siRNA was …
Improved siRNA/shRNA functionality by mismatched duplex
siRNA (small interfering RNA) and shRNA (small hairpin RNA) are powerful and commonly
used tools in biomedical research. Currently, siRNAs are generally designed as two 21 nt …
used tools in biomedical research. Currently, siRNAs are generally designed as two 21 nt …
Dual miRNases for triple incision of miRNA target: design concept and catalytic performance
O Patutina, D Chiglintseva, E Bichenkova, S Gaponova… - Molecules, 2020 - mdpi.com
Irreversible destruction of disease-associated regulatory RNA sequences offers exciting
opportunities for safe and powerful therapeutic interventions against human …
opportunities for safe and powerful therapeutic interventions against human …
Chemical modifications in RNA interference and CRISPR/Cas genome editing reagents
KA Lennox, MA Behlke - RNA Interference and CRISPR Technologies …, 2020 - Springer
Chemically modified oligonucleotides (ONs) are routinely used in the laboratory to assess
gene function, and clinical advances are rapidly progressing as continual efforts are being …
gene function, and clinical advances are rapidly progressing as continual efforts are being …