Chromatin and transcription in yeast

OJ Rando, F Winston - Genetics, 2012 - academic.oup.com
Understanding the mechanisms by which chromatin structure controls eukaryotic
transcription has been an intense area of investigation for the past 25 years. Many of the key …

[HTML][HTML] mRNA export and the TREX complex

J Katahira - Biochimica et Biophysica Acta (BBA)-Gene Regulatory …, 2012 - Elsevier
Over the past few decades, we have learned that eukaryotes have evolved sophisticated
means to coordinate the nuclear export of mRNAs with different steps of gene expression …

Exposed hydrophobicity is a key determinant of nuclear quality control degradation

EK Fredrickson, JC Rosenbaum… - Molecular biology of …, 2011 - Am Soc Cell Biol
Protein quality control (PQC) degradation protects the cell by preventing the toxic
accumulation of misfolded proteins. In eukaryotes, PQC degradation is primarily achieved by …

A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription

MA Sdano, JM Fulcher, S Palani… - Elife, 2017 - elifesciences.org
We determined that the tandem SH2 domain of S. cerevisiae Spt6 binds the linker region of
the RNA polymerase II subunit Rpb1 rather than the expected sites in its heptad repeat …

Regulation of histone gene transcription in yeast

CF Kurat, J Recht, E Radovani, T Durbic… - Cellular and molecular …, 2014 - Springer
Histones are the primary protein component of chromatin, the mixture of DNA and proteins
that packages the genetic material in eukaryotes. Large amounts of histones are required …

Control of chromatin structure by spt6: different consequences in coding and regulatory regions

I Ivanovska, PÉ Jacques, OJ Rando… - … and cellular biology, 2011 - Am Soc Microbiol
Spt6 is a highly conserved factor required for normal transcription and chromatin structure.
To gain new insights into the roles of Spt6, we measured nucleosome occupancy along …

Sharing the load: Mex67–Mtr2 cofunctions with Los1 in primary tRNA nuclear export

K Chatterjee, S Majumder, Y Wan, V Shah… - Genes & …, 2017 - genesdev.cshlp.org
Eukaryotic transfer RNAs (tRNAs) are exported from the nucleus, their site of synthesis, to
the cytoplasm, their site of function for protein synthesis. The evolutionarily conserved β …

Selective destruction of abnormal proteins by ubiquitin-mediated protein quality control degradation

EK Fredrickson, RG Gardner - Seminars in cell & developmental biology, 2012 - Elsevier
Misfolded proteins are continuously produced in the cell and present an escalating
detriment to cellular physiology if not managed effectively. As such, all organisms have …

Assembly of an export-competent mRNP is needed for efficient release of the 3′-end processing complex after polyadenylation

X Qu, S Lykke-Andersen, T Nasser… - … and cellular biology, 2009 - Am Soc Microbiol
Before polyadenylated mRNA is exported from the nucleus, the 3′-end processing complex
is removed by a poorly described mechanism. In this study, we asked whether factors …

Genome walking in eukaryotes

C Leoni, M Volpicella, F De Leo, R Gallerani… - The FEBS …, 2011 - Wiley Online Library
Genome walking is a molecular procedure for the direct identification of nucleotide
sequences from purified genomes. The only requirement is the availability of a known …