Characterizing endogenous protein complexes with biological mass spectrometry
R Rogawski, M Sharon - Chemical reviews, 2021 - ACS Publications
Biological mass spectrometry (MS) encompasses a range of methods for characterizing
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …
Single-pot, solid-phase-enhanced sample preparation for proteomics experiments
A critical step in proteomics analysis is the optimal extraction and processing of protein
material to ensure the highest sensitivity in downstream detection. Achieving this requires a …
material to ensure the highest sensitivity in downstream detection. Achieving this requires a …
Extending the compatibility of the SP3 paramagnetic bead processing approach for proteomics
The diversity in protein and peptide biochemistry necessitates robust protocols and reagents
for efficiently handling and enriching these molecules prior to analysis with mass …
for efficiently handling and enriching these molecules prior to analysis with mass …
Emerging technologies in adipose tissue research
Technologies are transforming the understanding of adipose tissue as a complex and
dynamic tissue that plays a critical role in energy homoeostasis and metabolic health. This …
dynamic tissue that plays a critical role in energy homoeostasis and metabolic health. This …
Ultrafast and reproducible proteomics from small amounts of heart tissue enabled by Azo and timsTOF pro
Global bottom-up mass spectrometry (MS)-based proteomics is widely used for protein
identification and quantification to achieve a comprehensive understanding of the …
identification and quantification to achieve a comprehensive understanding of the …
Evaluation of six sample preparation procedures for qualitative and quantitative proteomics analysis of milk fat globule membrane
Y Yang, E Anderson, S Zhang - Electrophoresis, 2018 - Wiley Online Library
Proteomic analysis of membrane proteins is challenged by the proteins solubility and
detergent incompatibility with MS analysis. No single perfect protocol can be used to …
detergent incompatibility with MS analysis. No single perfect protocol can be used to …
The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation
MER Maitland, G Onea, CA Chiasson, XU Wang… - Scientific reports, 2019 - nature.com
The multi-subunit C-terminal to LisH (CTLH) complex is the mammalian homologue of the
yeast Gid E3 ubiquitin ligase complex. In this study, we investigated the human CTLH …
yeast Gid E3 ubiquitin ligase complex. In this study, we investigated the human CTLH …
[HTML][HTML] Proteomic analysis of ubiquitination substrates reveals a CTLH E3 ligase complex‐dependent regulation of glycolysis
Ubiquitination is an essential post‐translational modification that regulates protein stability
or function. Its substrate specificity is dictated by various E3 ligases. The human C‐terminal …
or function. Its substrate specificity is dictated by various E3 ligases. The human C‐terminal …
Distinct nuclear and cytoplasmic assemblies and interactomes of the mammalian CTLH E3 ligase complex
G Onea, MER Maitland, X Wang… - Journal of Cell …, 2022 - journals.biologists.com
The C-terminal to LisH (CTLH) complex is a newly discovered multi-subunit E3 ubiquitin
ligase and its cellular functions are poorly characterized. Although some CTLH subunits …
ligase and its cellular functions are poorly characterized. Although some CTLH subunits …
Interplay between β-propeller subunits WDR26 and muskelin regulates the CTLH E3 ligase supramolecular complex
The Pro/N-degron recognizing C-terminal to LisH (CTLH) complex is an E3 ligase of
emerging interest in the developmental biology field and for targeted protein degradation …
emerging interest in the developmental biology field and for targeted protein degradation …