Is passive transmission of non-viral vectors through artificial insemination of sperm-DNA mixtures sufficient for chicken transgenesis?

S Chaparian, A Abdulahnejad, F Rashidi… - … of Reproduction and …, 2016 - jstage.jst.go.jp
DNA uptake in the post-acrosomal region of the spermatozoa takes place exclusively in
immotile spermatozoa that are naturally unable to fertilize eggs. The present study aimed to …

Developing a puncture-free in ovo chicken transfection strategy based on bypassing albumen nucleases

HR Amini, A Pakdel, HM Shahr-Babak, S Eghbalsaied - Theriogenology, 2017 - Elsevier
Chicken is a dual-purpose animal important from both agricultural and medical aspects.
Even though significant improvements have been made in chicken transgenesis …

Expression of fluorescent reporter protein was not obtained in ovine embryos produced through in vitro fertilization-sperm mediated gene transfer (IVF-SMGT)

R Hasanzadeh, M Zandi, MR Sanjabi, KH Pajooh… - Small Ruminant …, 2016 - Elsevier
Sperm mediated gene transfer (SMGT) has been reported to be a powerful tool for
producing transgenic animals on a mass scale using spermatozoa as vectors for exogenous …