Developing Gram-negative bacteria for the secretion of heterologous proteins

LA Burdette, SA Leach, HT Wong… - Microbial cell …, 2018 - Springer
Gram-negative bacteria are attractive hosts for recombinant protein production because they
are fast growing, easy to manipulate, and genetically stable in large cultures. However, the …

Type I secretion system—it takes three and a substrate

K Kanonenberg, O Spitz, IN Erenburg… - FEMS microbiology …, 2018 - academic.oup.com
Type I secretion systems are widespread in Gram-negative bacteria and mediate the one-
step translocation of a large variety of proteins serving for diverse purposes, including …

Efficient extracellular laccase secretion via bio-designed secretory apparatuses to enhance bacterial utilization of recalcitrant lignin

L Cao, L Lin, H Sui, H Wang, Z Zhang, N Jiao… - Green Chemistry, 2021 - pubs.rsc.org
Microbial-driven lignin conversion has a significant impact on the biogeochemistry of global
ecosystems and biomass utilization. During this process, secretion of extracellular …

Biotechnological applications of type 1 secretion systems

Z Pourhassan, SHJ Smits, JH Ahn, L Schmitt - Biotechnology Advances, 2021 - Elsevier
Bacteria have evolved a diverse range of secretion systems to export different substrates
across their cell envelope. Although secretion of proteins into the extracellular space could …

Export of Diverse and Bioactive Small Proteins through a Type I Secretion System

SY Kim, JK Parker, M Gonzalez-Magaldi… - Applied and …, 2023 - Am Soc Microbiol
Small proteins perform a diverse array of functions, from microbial competition, to endocrine
signaling, to building biomaterials. Microbial systems that can produce recombinant small …

Engineering high-yield biopolymer secretion creates an extracellular protein matrix for living materials

MT Orozco-Hidalgo, M Charrier, N Tjahjono… - Msystems, 2021 - Am Soc Microbiol
The bacterial extracellular matrix forms autonomously, giving rise to complex material
properties and multicellular behaviors. Synthetic matrix analogues can replicate these …

Emerging Landscape of Supercharged Proteins and Peptides for Drug Delivery

L Wang, J Geng, H Wang - ACS Pharmacology & Translational …, 2024 - ACS Publications
Although groundbreaking biotechnological techniques such as gene editing have
significantly progressed, the effective and targeted transport of therapeutic agents into host …

Generalized approach towards secretion-based protein production via neutralization of secretion-preventing cationic substrate residues

H Byun, J Park, BU Fabia, J Bingwa… - International Journal of …, 2022 - mdpi.com
Many heterologous proteins can be secreted by bacterial ATP-binding cassette (ABC)
transporters, provided that they are fused with the C-terminal signal sequence, but some …

PySupercharge: a python algorithm for enabling ABC transporter bacterial secretion of all proteins through amino acid mutation

Y Kim, D Kim, NM Hieu, H Byun, JH Ahn - Microbial Cell Factories, 2024 - Springer
Background The process of producing proteins in bacterial systems and secreting them
through ATP-binding cassette (ABC) transporters is an area that has been actively …

Suspension cell secretome of the grain legume Lathyrus sativus (grasspea) reveals roles in plant development and defense responses

D Rathi, JK Verma, S Chakraborty, N Chakraborty - Phytochemistry, 2022 - Elsevier
Plant secretomics has been especially important in understanding the molecular basis of
plant development, stress resistance and biomarker discovery. In addition to sharing a …