D-allulose, a versatile rare sugar: recent biotechnological advances and challenges

W Zhang, D Chen, J Chen, W Xu, Q Chen… - Critical Reviews in …, 2023 - Taylor & Francis
D-Allulose is the C-3 epimer of D-fructose, and widely regarded as a promising substitute for
sucrose. It's an excellent low-calorie sweetener, with 70% sweetness of sucrose, 0.4 kcal/g …

Harnessing the biocatalytic attributes and applied perspectives of nanoengineered laccases—A review

M Bilal, SS Ashraf, J Cui, WY Lou, M Franco… - International journal of …, 2021 - Elsevier
In the recent past, numerous new types of nanostructured carriers, as support matrices, have
been engineered to advance the traditional enzyme immobilization strategies. The current …

D-allulose 3-epimerase for low-calorie D-allulose synthesis: microbial production, characterization, and applications

X Xie, C Li, X Ban, H Yang, Z Li - Critical Reviews in Biotechnology, 2024 - Taylor & Francis
D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable
physiochemical properties and special physiological functions, which displays promising …

Nanotechnology and enzyme immobilization: a review

MA Oke, SA Ojo, SA Fasiku, EA Adebayo - Nanotechnology, 2023 - iopscience.iop.org
The synergy between enzymes and nanotechnology (nano-biocatalysts) has created some
of the most promising biomaterials fabricated by synergistically incorporating advanced …

Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose

X Gao, C Wei, H Qi, C Li, F Lu, HM Qin - Food Chemistry, 2023 - Elsevier
D-Allulose, as low-calorie rare sugar, possessed several notable biological activities and
was biosynthesized by D-allulose 3-epimerase (DAEase). Here, CcDAE from Clostridium …

Two-step biosynthesis of D-allulose via a multienzyme cascade for the bioconversion of fruit juices

C Li, L Li, Z Feng, L Guan, F Lu, HM Qin - Food Chemistry, 2021 - Elsevier
Abstract d-Allulose, a low-calorie rare sugar with potential as sucrose substitute for
diabetics, can be produced using d-allulose 3-epimerase (DAE). Here, we characterized a …

Advances in the biosynthesis of D-allulose

Y Zhang, Z Zhou, H Luan, X Zhang, M Liu… - World Journal of …, 2024 - Springer
D-allulose is a rare monosaccharide and a C-3 epimer of D-fructose. It has physiological
functions, such as antihyperglycemic, obesity-preventing, neuroprotective, and reactive …

Engineering Escherichia coli for d-Allulose Production from d-Fructose by Fermentation

Q Guo, LJ Zheng, X Luo, XQ Gao, CY Liu… - Journal of Agricultural …, 2021 - ACS Publications
d-Allulose is considered an ideal alternative to sucrose and has shown tremendous
application potential in many fields. Recently, most efforts on production of d-allulose have …

Surface activation of hierarchically porous diatomite modified with polyethyleneimine for immobilizing d-allulose 3-epimerase

Z Xiao, Z Zhao, B Jiang, J Chen - Food Bioscience, 2023 - Elsevier
Abstract d-Allulose, a low-calorie functional sweetener popular among the obese
population, can be biosynthesized using d-allulose 3-epimerase (DAE). However, there are …

Green preparation of covalently co-immobilized multienzymes on silica nanoparticles for clean production of reducing sugar from lignocellulosic biomass

Y Lin, W Jin, L Cai, X Liu, Y Qiu, G Zhang - Journal of Cleaner Production, 2021 - Elsevier
Multienzyme co-immobilization has garnered increasing interest in biomass conversion due
to the green catalytic properties. However, the conventional strategies for covalent co …