The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …

CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

A series of recent discoveries harnessing the adaptive immune system of prokaryotes to
perform targeted genome editing is having a transformative influence across the biological …

The powerful CRISPR genome editing system is hindered by its off-target effects, and
existing computational tools achieved limited performance in genome-wide off-target …

Although several high-fidelity SpCas9 variants have been reported, it has been observed
that this increased specificity is associated with reduced on-target activity, limiting the …

The emergence and development of antibiotic resistance in bacteria is a serious threat to
global public health. Antibiotic resistance genes (ARGs) are often located on mobile genetic …

CRISPR technology is a universal tool for genome engineering that has revolutionized
biotechnology. Recently identified unique CRISPR/Cas systems, as well as re-engineered …

Secondary natural products (NPs) are a rich source for drug discovery. However, the low
abundance of NPs makes their extraction from nature inefficient, while chemical synthesis is …

The clustered regularly interspaced short palindromic repeat (CRISPR) genome-editing
revolution established the beginning of a new era in life sciences. Here, we review the role …