Fluorescence-activated droplet sorting (FADS) has emerged as a powerful tool for ultrahigh-
throughput screening of enzymes, metabolites, and antibodies. Fluorescence coupling …

Highlights•Miniaturization is the key towards microbial single-cell MS.•Smart integration of
cell handling, incubation, and analyte ionization is mandatory.•Volatile cell-compatible …

Synthetic biology relies on engineering cells to have desirable properties, such as the
production of select chemicals. A bottleneck in engineering methods is often the need to …

Dose–response, or “conforming” behavior, increases confidence in a screening hit's
authenticity. Here, we demonstrate dose–response solid-phase DNA-encoded library (DEL) …

Directed evolution is a powerful technique for creating biomolecules such as proteins and
nucleic acids with tailor-made properties for therapeutic and industrial applications by …

Microdroplets are compartments made in the laboratory that allow the miniaturisation of
chemical and biological experiments to the femto-to picolitre scale, replacing the classical …

Droplet microfluidics marks a significant advancement from the traditional continuous flow
systems by utilizing two non-mixing liquid phases to generate droplets for further …

The cycle time of a standard liquid chromatography (LC) system is the sum of the time for the
chromatographic run and the autosampler injection sequence. Although LC separation …

Abstract Analysis of an emulsion in its original dispersed condition is quite important for
quality assessment and quality control. In the present study, the practical experimental …

Enzyme engineering and discovery are crucial for a future sustainable bioeconomy, and
harvesting new biocatalysts from large libraries through directed evolution or functional …