Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies

S MacAulay, AR Ellison, P Kille… - Reviews in …, 2022 - Wiley Online Library
Early and accurate diagnosis is key to mitigating the impact of infectious diseases, along
with efficient surveillance. This however is particularly challenging in aquatic environments …

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

JA Cardona-Ospina, MF Villalba-Miranda… - Preventive veterinary …, 2019 - Elsevier
BackgroundDetection and characterization of viral RNA pathogens from fieldwork are
challenging due to the instability of the RNA molecule. FTA cards® have proved useful for …

Transcriptomic analysis of fish hosts responses to nervous necrosis virus

DK Toubanaki, A Efstathiou, E Karagouni - Pathogens, 2022 - mdpi.com
Nervous necrosis virus (NNV) has been responsible for mass mortalities in the aquaculture
industry worldwide, with great economic and environmental impact. The present review aims …

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future …

JV Samsonova, NY Saushkin, AP Osipov - Veterinary Research …, 2022 - Springer
Abstract Dried Blood Spots (DBS) technology has become a valuable tool in medical
studies, however, in veterinary and biological research DBS technology applications are still …

Low-cost and rapid method of DNA extraction from scaled fish blood and skin mucus

L Gui, X Li, S Lin, Y Zhao, P Lin, B Wang, R Tang… - Viruses, 2022 - mdpi.com
PCR-based DNA amplification has been one of the major methods in aquaculture research
for decades, although its use outside the modern laboratory environment is limited due to …

Co-infection of viral nervous necrosis (VNN) and bacterial infection outbreak in cage cultured cobia fingerlings

P Rameshkumar, C Balachandran, S Vairamuthu… - Aquaculture, 2024 - Elsevier
Two disease outbreaks were encountered in cage cultured cobia fingerlings with the
mortality of 36.80% and 83.70% during summer month at Palk Bay and Gulf of Mannar …

FTA® card tool for sampling and rapid diagnosis of bacterial diseases from rainbow trout (Oncorhynchus mykiss) tissue

IT Çağatay - Aquaculture International, 2022 - Springer
The collection of farmed fish samples and subsequent diagnosis of microbial pathogens can
be difficult and time-consuming procedure under field conditions. Correct diagnosis and …

A new solid matrix for preservation of viral nucleic acid from clinical specimens at ambient temperature

T Cromeans, N Jothikumar, J Lee, N Collins… - Journal of virological …, 2019 - Elsevier
Stabilizing paper matrix methods for retaining nucleic acid from inactivated clinical
specimens offer a solution for molecular diagnostics when specimens may be stored or …

Development of simplified and rapid nucleic acid release protocol for PCR based detection of Potato viruses

B Raigond, A Verma, T Kochhar, S Roach, S Sharma… - Phytoparasitica, 2018 - Springer
A simple, cost-effective and rapid viral nucleic acid release (NAR) buffer suitable for RT-PCR
based diagnostic assay was developed for the detection of potato viruses. The NAR buffer …

[PDF][PDF] Evaluation of flinders technology associates cards as a non-lethal sampling device for molecular diagnosis of Betanodavirus in Asian seabass, Lates calcarifer …

K Kirti, PE Praveena, T Bhuvaneswari… - Virol Curr …, 2019 - researchgate.net
This study aimed to evaluate the utility and efficiency of the Flinders Technology Associates
(FTA®) Elute Card (Whatman®) as a sampling device and storage platform for RNA from …