Enzymatic production of prebiotic oligosaccharides

C Vera, A Illanes, C Guerrero - Current Opinion in Food Science, 2021 - Elsevier
Highlights•Non-digestible oligosaccharides (NDOs) are the major class of prebiotic
ingredients.•Synthesis of NDOs allows the upgrade of underutilized carbohydrates.•Enzyme …

[HTML][HTML] The successful synthesis of industrial isomaltooligosaccharides lies in the use of transglycosylating α-glucosidases: A review

S Kumar, T Tissopi, S Mutturi - Carbohydrate Polymer Technologies and …, 2023 - Elsevier
Consumption of oligosaccharides which can evade host digestive enzymes but can be
selectively taken-up by populations of bacteria native to the host colon in situ, improves the …

Production of a Series of Long-Chain Isomaltooligosaccharides from Maltose by Bacillus subtilis AP-1 and Associated Prebiotic Properties

S Tiangpook, S Nhim, P Prangthip, P Pason… - Foods, 2023 - mdpi.com
Bacillus subtilis strain AP-1, which produces α-glucosidase with transglucosidase activity,
was used to produce a series of long-chain isomaltooligosaccharides (IMOs) with degree of …

Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification

W Guo, J Yang, T Huang, D Liu, Q Liu, J Li… - Biotechnology for …, 2021 - Springer
Background Starch is one of the most important renewable polysaccharides in nature for
production of bio-ethanol. The starch saccharification step facilitates the depolymerization of …

Expression of a novel α-glucosidase from Aspergillus neoniger in Pichia pastoris and its efficient recovery for synthesis of isomaltooligosaccharides

S Kumar, S Mutturi - Enzyme and Microbial Technology, 2020 - Elsevier
A gene conferring α-glucosidase (AG) with high transglycosylation activity from Aspergillus
neoniger (a non-niger strain belonging to section Nigri) was cloned and expressed in Pichia …

Surface display of novel transglycosylating α-glucosidase from Aspergillus neoniger on Pichia pastoris for synthesis of isomaltooligosaccharides

T Tissopi, S Kumar, A Sadhu, S Mutturi - Biochemical Engineering Journal, 2022 - Elsevier
In the present study, α-glucosidase gene of Aspergillus neoniger, agdA, was fused with
glycosylphosphatidylinositol (GPI)-anchored glycoprotein gene of Pichia pastoris, GCW61 at …

Desirability function for optimization of the synthesis of high-panose isomaltooligosaccharides from maltose catalyzed by a novel commercial enzyme preparation from …

C Berrocal, H Chico, E Carranza, R Vega - Biochemical Engineering …, 2021 - Elsevier
Isomaltooligosaccharides (IMOs) have wide applications as functional food ingredients, but
their increasing demand has intensified the need to produce IMOs enriched with a specific …

Biochemical characterization of glycoside hydrolase family 31 α-glucosidases from Myceliophthora thermophila for α-glucooligosaccharide synthesis

Y Fang, M Dong, SS van Leeuwen, L Dijkhuizen… - International Journal of …, 2023 - Elsevier
The transglucosidase activity of GH31 α-glucosidases is employed to catalyze the synthesis
of prebiotic isomaltooligosaccharides (IMOs) using the malt syrup prepared from starch as …

Insights into the transglucosylation activity of α-glucosidase from Schwanniomyces occidentalis

Z Merdzo, E Narmontaite, JL Gonzalez-Alfonso… - Applied Microbiology …, 2024 - Springer
The α-glucosidase from Schwanniomyces occidentalis (GAM1p) was expressed in
Komagataella phaffii to about 70 mg/L, and its transferase activity studied in detail. Several …

Changes in polyphenols composition and antioxidative properties of hemp (Cannabis sativa L.) inflorescences pretreated by Ganoderma lucidum

Z Zhu, Y Wang, D Lin, G Liu, C Cao, W Gong… - Industrial Crops and …, 2023 - Elsevier
Highlights•G. lucidum is the optimal fungi to pretreat hemp inflorescences.•The antioxidative
activities of hemp inflorescences increased up to 3.92 fold.•G. lucidum exerts significant …