Simple indolizidine and quinolizidine alkaloids

JP Michael - The alkaloids: Chemistry and biology, 2016 - Elsevier
This review of simple indolizidine and quinolizidine alkaloids (ie, those in which the parent
bicyclic systems are in general not embedded in polycyclic arrays) is an update of the …

Enzymatic cleavage of glycosidic bonds: strategies on how to set up and control a QM/MM metadynamics simulation

L Raich, A Nin-Hill, A Ardèvol, C Rovira - Methods in enzymology, 2016 - Elsevier
Carbohydrates play crucial roles in many biological processes, from cell–cell adhesion to
chemical signaling. Their complexity and diversity, related to α/β anomeric configuration …

Molecular mechanism of the glycosylation step catalyzed by Golgi α-mannosidase II: a QM/MM metadynamics investigation

L Petersen, A Ardevol, C Rovira… - Journal of the American …, 2010 - ACS Publications
Golgi α-mannosidase II (GMII), a member of glycoside hydrolase family 38, cleaves two
mannosyl residues from GlcNAcMan5GlcNAc2 as part of the N-linked glycosylation …

Charge-switchable gold nanoparticles for enhanced enzymatic thermostability

S Shankar, SK Soni, HK Daima… - Physical Chemistry …, 2015 - pubs.rsc.org
This study illustrates a facile strategy for efficient immobilization of enzymes on a metal
nanoparticle surface. The strategy proposed here enables the enzymatic activity to be …

Characterization of Solitalea canadensis α-mannosidase with specific activity towards α1, 3-Mannosidic linkages

FF Liu, M Wang, GH Ma, A Kulinich, L Liu… - Carbohydrate …, 2024 - Elsevier
A recombinant exo-α-mannosidase from Solitalea canadensis (Sc3Man) has been
characterized to exhibit strict specificity for hydrolyzing α1, 3-mannosidic linkages located at …

Catalytic Reaction Mechanism of Bacterial GH92 α‐1, 2‐Mannosidase: A QM/MM Metadynamics Study

M Sagiroglugil, F Yasar - ChemPhysChem, 2023 - Wiley Online Library
The catalytic mechanism of a‐dependent family 92‐mannosidase, which is abundantly
present in human gut flora and malfunctions leading to the lysosomal storage disease α …

Sequential processing of mannose-containing glycans by two α-mannosidases from Solitalea canadensis

FF Liu, A Kulinich, YM Du, L Liu, J Voglmeir - Glycoconjugate journal, 2016 - Springer
Two putative α-mannosidase genes isolated from the rather unexplored soil bacterium
Solitalea canadensis were cloned and biochemically characterised. Both recombinant …

Biophysicochemical Characterization of an Alkaline Protease from Beauveria sp. MTCC 5184 with Multiple Applications

S Shankar, RS Laxman - Applied biochemistry and biotechnology, 2015 - Springer
This study illustrates the biophysicochemical properties of an alkaline protease, BAP
(Beauveria sp. alkaline protease) from Beauveria sp. MTCC 5184. This protease exhibited …

Jack bean α-mannosidase (Jbα-man): Tolerance to alkali, chelating and reducing agents and energetics of catalysis and inhibition

A Kumar, SM Gaikwad - International journal of biological macromolecules, 2011 - Elsevier
Investigations of the catalytic and structural transitions of jack bean α-mannosidase (Jbα-
man) are described in the present paper. The enzyme was maximally stable at pH 5.0; …

Biochemical characterization of recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) from Leucaena leucocephala

P Sonawane, RK Vishwakarma, BM Khan - International journal of …, 2013 - Elsevier
Recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) protein from Leucaena leucocephala
was overexpressed in Escherichia coli BL21 (DE3) strain and purified to apparent …