Dance with spins: site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy directly inside cells

A Pierro, M Drescher - Chemical Communications, 2023 - pubs.rsc.org
Depicting how biomolecules move and interact within their physiological environment is one
of the hottest topics of structural biology. This Feature Article gives an overview of the most …

Orthogonal spin labeling and pulsed dipolar spectroscopy for protein studies

L Galazzo, M Teucher, E Bordignon - Methods in Enzymology, 2022 - Elsevier
Different types of spin labels are currently available for structural studies of biomolecules
both in vitro and in cells using Electron Paramagnetic Resonance (EPR) and pulse dipolar …

Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy

A Bertran, L Morbiato, J Sawyer… - Journal of the …, 2023 - ACS Publications
To carry out reliable and comprehensive structural investigations, the exploitation of different
complementary techniques is required. Here, we report that dual triplet-spin/fluorescent …

Increasing the Modulation Depth of GdIII-Based Pulsed Dipolar EPR Spectroscopy (PDS) with Porphyrin–GdIII Laser-Induced Magnetic Dipole Spectroscopy

A Scherer, X Yao, M Qi, M Wiedmaier… - The Journal of …, 2022 - ACS Publications
Distance determination with pulsed EPR has become an important technique for the
structural investigation of biomacromolecules, with double electron–electron resonance …

Fullerene-based triplet spin labels: methodology aspects for pulsed dipolar EPR spectroscopy

IO Timofeev, LV Politanskaya, EV Tretyakov… - Physical Chemistry …, 2022 - pubs.rsc.org
Triplet states of photoexcited organic molecules are promising spin labels with advanced
spectroscopic properties for pulsed dipolar electron paramagnetic resonance (PD EPR) …

Orientation-selective and frequency-correlated light-induced pulsed dipolar spectroscopy

AM Bowen, A Bertran, KB Henbest… - The Journal of …, 2021 - ACS Publications
We explore the potential of orientation-resolved pulsed dipolar spectroscopy (PDS) in light-
induced versions of the experiment. The use of triplets as spin-active moieties for PDS offers …

Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy

A Bertran, L Morbiato, S Aquilia, L Gabbatore… - Molecules, 2022 - mdpi.com
We present a new photoswitchable spin label for light-induced pulsed electron
paramagnetic resonance dipolar spectroscopy (LiPDS), the photoexcited triplet state of …

Sensitivity optimization in pulse EPR experiments with photo-labels by multiple-echo-integrated dynamical decoupling

NE Sannikova, AR Melnikov, SL Veber… - Physical Chemistry …, 2023 - pubs.rsc.org
Photo-excited triplet states represent a new class of spin labels in pulse electron
paramagnetic resonance (EPR), attracting increasing attention because of their unique …

Light-induced pulsed dipolar EPR spectroscopy for distance and orientation analysis

A Bertran, A Barbon, AM Bowen, M Di Valentin - Methods in Enzymology, 2022 - Elsevier
Measuring distances in biology at the molecular level is of great importance for
understanding the structure and function of proteins, nucleic acids and other biological …

Revealing light-induced structural shifts in G-quadruplex-porphyrin complexes: a pulsed dipolar EPR study

NE Sannikova, MI Kolokolov, TA Khlynova… - Physical Chemistry …, 2023 - pubs.rsc.org
The binding of G-quadruplex structures (G4s) with photosensitizers is of considerable
importance in medicinal chemistry and drug discovery due to their promising potential in …