Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods,
together with associated computational tools and the growing availability of public data …

Over 17 and 160 types of chemical modifications have been identified in DNA and RNA,
respectively. The interest in understanding the various biological functions of DNA and RNA …

Mapping single-cell sequencing profiles to comprehensive reference datasets provides a
powerful alternative to unsupervised analysis. However, most reference datasets are …

Technological advances have enabled the profiling of multiple molecular layers at single-
cell resolution, assaying cells from multiple samples or conditions. Consequently, there is a …

Formation of the three primary germ layers during gastrulation is an essential step in the
establishment of the vertebrate body plan and is associated with major transcriptional …

Modulation of chromatin structure via histone modification is a major epigenetic mechanism
and regulator of gene expression. However, the contribution of chromatin features to tumor …

Parallel single-cell sequencing protocols represent powerful methods for investigating
regulatory relationships, including epigenome-transcriptome interactions. Here, we report a …

The “epigenetics” concept was first described in 1942. Thus far, chemical modifications on
histones, DNA, and RNA have emerged as three important building blocks of epigenetic …

Bisulfite sequencing (BS-seq) to detect 5-methylcytosine (5mC) is limited by lengthy reaction
times, severe DNA damage, overestimation of 5mC level and incomplete C-to-U conversion …

We present a highly scalable assay for whole-genome methylation profiling of single cells.
We use our approach, single-cell combinatorial indexing for methylation analysis (sci-MET) …