Soil-specific limitations for access and analysis of soil microbial communities by metagenomics

N Lombard, E Prestat, JD van Elsas… - FEMS microbiology …, 2011 - academic.oup.com
Metagenomics approaches represent an important way to acquire information on the
microbial communities present in complex environments like soil. However, to what extent …

Molecular Phylogeny of the Genus Frankia and Related Genera and Emendation of the Family Frankiaceae

P Normand, S Orso, B Cournoyer… - … of Systematic and …, 1996 - microbiologyresearch.org
The members of the actinomycete genus Frankia are nitrogen-fixing symbionts of many
species of woody dicotyledonous plants belonging to eight families. Several strains isolated …

Extraction of DNA from soil

P Robe, R Nalin, C Capellano, TM Vogel… - European Journal of Soil …, 2003 - Elsevier
There is an increased interest in the extraction of nucleic acids from various environmental
samples, since molecular techniques allow less biased access to a greater portion of …

Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction

C Picard, C Ponsonnet, E Paget… - Applied and …, 1992 - Am Soc Microbiol
In order to develop a rapid and specific detection test for bacteria in soil, we improved a
method based on the polymerase chain reaction (PCR). Each step of the protocol, including …

Detection and counting of Nitrobacter populations in soil by PCR

V Degrange, R Bardin - Applied and Environmental Microbiology, 1995 - Am Soc Microbiol
Although the biological conversion of nitrite to nitrate is a well-known process, studies of
Nitrobacter populations are hindered by their physiological characteristics. This report …

Advances in actinorhizal symbiosis: host plant-Frankia interactions, biology, and applications in arid land reclamation. A review

J Schwencke, M Carú - Arid Land Research and Management, 2001 - Taylor & Francis
Symbiotic association of the N 2-fixing actinomycete Frankia with the roots of more than 200
tree species from 24 genera of 8 families of angiosperms has been studied since 1829. The …

Frankia genus-specific characterization by polymerase chain reaction

P Simonet, MC Grosjean, AK Misra… - Applied and …, 1991 - Am Soc Microbiol
The polymerase chain reaction (PCR) is an in vitro procedure for primer-directed enzymatic
amplification of specific template nucleic acid sequences. In order to determine whether a …

Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer

E Navarro, P Simonet, P Normand, R Bardin - Archives of Microbiology, 1992 - Springer
DNA sequences from the intergenic spacer (IGS) region of the ribosomal operon were
amplified by the polymerase chain reaction (PCR) technique using two primers derived from …

Deoxyribonucleic Acid Relatedness among Members of the Genus Frankia

MP Fernandez, H Meugnier… - … of Systematic and …, 1989 - microbiologyresearch.org
The diversity among 43 isolates of the genus Frankia was studied by determining levels of
deoxyribonucleic acid relatedness (SI nuclease method) and DNA base compositions. The …

Structural and functional comparison of Frankia root hair deforming factor and rhizobia Nod factor

H Cérémonie, F Debellé… - Canadian Journal of …, 1999 - cdnsciencepub.com
Les processus infectieux des symbioses Frankia-Alnus et Rhizobium-légumineuses
présentent de fortes similarités, laissant supposer l'existence d'analogies entre le facteur …