A new era in functional genomics screens
L Przybyla, LA Gilbert - Nature Reviews Genetics, 2022 - nature.com
The past 25 years of genomics research first revealed which genes are encoded by the
human genome and then a detailed catalogue of human genome variation associated with …
human genome and then a detailed catalogue of human genome variation associated with …
[HTML][HTML] Multimodal analysis of composition and spatial architecture in human squamous cell carcinoma
To define the cellular composition and architecture of cutaneous squamous cell carcinoma
(cSCC), we combined single-cell RNA sequencing with spatial transcriptomics and …
(cSCC), we combined single-cell RNA sequencing with spatial transcriptomics and …
A non-canonical tricarboxylic acid cycle underlies cellular identity
The tricarboxylic acid (TCA) cycle is a central hub of cellular metabolism, oxidizing nutrients
to generate reducing equivalents for energy production and critical metabolites for …
to generate reducing equivalents for energy production and critical metabolites for …
[HTML][HTML] Dual proteome-scale networks reveal cell-specific remodeling of the human interactome
Thousands of interactions assemble proteins into modules that impart spatial and functional
organization to the cellular proteome. Through affinity-purification mass spectrometry, we …
organization to the cellular proteome. Through affinity-purification mass spectrometry, we …
Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability
Z Li, L Ferguson, KK Deol, MA Roberts… - Nature chemical …, 2022 - nature.com
The selenoprotein glutathione peroxidase 4 (GPX4) prevents ferroptosis by converting lipid
peroxides into nontoxic lipid alcohols. GPX4 has emerged as a promising therapeutic target …
peroxides into nontoxic lipid alcohols. GPX4 has emerged as a promising therapeutic target …
[HTML][HTML] Regulation of plasmalogen metabolism and traffic in mammals: The fog begins to lift
F Dorninger, ER Werner, J Berger… - Frontiers in Cell and …, 2022 - frontiersin.org
Due to their unique chemical structure, plasmalogens do not only exhibit distinct biophysical
and biochemical features, but require specialized pathways of biosynthesis and …
and biochemical features, but require specialized pathways of biosynthesis and …
Functional unknomics: Systematic screening of conserved genes of unknown function
JJ Rocha, SA Jayaram, TJ Stevens, N Muschalik… - PLoS …, 2023 - journals.plos.org
The human genome encodes approximately 20,000 proteins, many still uncharacterised. It
has become clear that scientific research tends to focus on well-studied proteins, leading to …
has become clear that scientific research tends to focus on well-studied proteins, leading to …
CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities
Cancer genomics studies have identified thousands of putative cancer driver genes.
Development of high-throughput and accurate models to define the functions of these genes …
Development of high-throughput and accurate models to define the functions of these genes …
Optimization of AsCas12a for combinatorial genetic screens in human cells
Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic
perturbations because they can process multiple guide RNAs expressed as a single …
perturbations because they can process multiple guide RNAs expressed as a single …
[PDF][PDF] The phenotypic landscape of essential human genes
Understanding the basis for cellular growth, proliferation, and function requires determining
the roles of essential genes in diverse cellular processes, including visualizing their …
the roles of essential genes in diverse cellular processes, including visualizing their …