Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Tutorial: guidance for quantitative confocal microscopy

J Jonkman, CM Brown, GD Wright, KI Anderson… - Nature protocols, 2020 - nature.com
When used appropriately, a confocal fluorescence microscope is an excellent tool for
making quantitative measurements in cells and tissues. The confocal microscope's ability to …

Soil plastisphere: exploration methods, influencing factors, and ecological insights

C Wang, L Wang, YS Ok, DCW Tsang, D Hou - Journal of hazardous …, 2022 - Elsevier
Microplastic (MP), an emerging contaminant, is globally prevalent and poses potential
environmental threats and ecological risks to both aquatic and terrestrial ecosystems. When …

Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens

AK Glaser, NP Reder, Y Chen, EF McCarty… - Nature biomedical …, 2017 - nature.com
For the 1.7 million patients per year in the US who receive a new cancer diagnosis,
treatment decisions are largely based on histopathological specimen examinations …

Tissue clearing to examine tumour complexity in three dimensions

J Almagro, HA Messal, M Zaw Thin… - Nature Reviews …, 2021 - nature.com
The visualization of whole organs and organisms through tissue clearing and fluorescence
volumetric imaging has revolutionized the way we look at biological samples. Its application …

Deep learning-based point-scanning super-resolution imaging

L Fang, F Monroe, SW Novak, L Kirk, CR Schiavon… - Nature …, 2021 - nature.com
Point-scanning imaging systems are among the most widely used tools for high-resolution
cellular and tissue imaging, benefiting from arbitrarily defined pixel sizes. The resolution …

Perspectives for applications of quantum imaging

M Gilaberte Basset, F Setzpfandt… - Laser & Photonics …, 2019 - Wiley Online Library
Quantum imaging is a multifaceted field of research that promises highly efficient imaging in
extreme spectral ranges as well as ultralow‐light microscopy. Since the first proof‐of …

Methods for physical characterization of phase-separated bodies and membrane-less organelles

DM Mitrea, B Chandra, MC Ferrolino, EB Gibbs… - Journal of molecular …, 2018 - Elsevier
Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …

Robotic micromanipulation: Fundamentals and applications

Z Zhang, X Wang, J Liu, C Dai… - Annual Review of Control …, 2019 - annualreviews.org
Robotic micromanipulation is a relatively young field. However, after three decades of
development and evolution, the fundamental physics; techniques for sensing, actuation, and …

Comparing repeatability and reproducibility of topographic measurement types directly using linear regression analyses of measured heights

K Peta, G Love, CA Brown - Precision Engineering, 2024 - Elsevier
This paper describes and illustrates a convenient, new method for checking repeatability
and reproducibility by direct comparisons of measured heights. Focus variation, confocal …