Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

Although great progress has been achieved regarding wheat genetic transformation
technology in the past decade,–, genotype dependency, the most impactful factor in wheat …

Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be
genetically modified due to limitations of the current gene delivery systems. Even for those …

Transformation in grass species has traditionally relied on immature embryos and has
therefore been limited to a few major Poaceae crops. Other transformation explants …

Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

Plant regeneration occurs when plants repair or replace damaged structures based on the
totipotency and pluripotency of their cells. Tissue culture is one of the most widely used …

The potential of genome editing to improve the agronomic performance of crops is often
limited by low plant regeneration efficiencies and few transformable genotypes. Here, we …

Plant gene editing is typically performed by delivering reagents such as Cas9 and single
guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole …

Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …