With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial
crystallography techniques for macromolecular structure determination have emerged …

BACKGROUND Conformational changes are essential for the correct functioning of
biological macromolecules. Time-resolved x-ray crystallography extends an extremely …

INTRODUCTION Photoenzymes are rare biocatalysts driven by absorption of a photon at
each catalytic cycle; they inspire development of artificial photoenzymes with valuable …

Highlights•Time-resolved serial femtosecond crystallography (tr-SFX) probes structure and
function in each sample.•X-ray free electron lasers (XFELS) provide atomic and electronic …

Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions.
In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a …

Photolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction
intermediates involved in the enzyme's catalytic cycle, we conducted a time-resolved …

High-intensity femtosecond pulses from an X-ray free-electron laser enable pump–probe
experiments for the investigation of electronic and nuclear changes during light-induced …

Careful selection of photocaging approaches is critical to achieve fast and well synchronized
reaction initiation and perform successful time-resolved structural biology experiments. This …

Vision is initiated by the rhodopsin family of light-sensitive G protein-coupled receptors
(GPCRs). A photon is absorbed by the 11-cis retinal chromophore of rhodopsin, which …

The structural dynamics of a molecule are determined by the underlying potential energy
landscape. Conical intersections are funnels connecting otherwise separate potential …