Microbial enzymes for the recycling of recalcitrant petroleum‐based plastics: how far are we?

R Wei, W Zimmermann - Microbial biotechnology, 2017 - Wiley Online Library
Petroleum‐based plastics have replaced many natural materials in their former applications.
With their excellent properties, they have found widespread uses in almost every area of …

Cutinase: characteristics, preparation, and application

S Chen, L Su, J Chen, J Wu - Biotechnology advances, 2013 - Elsevier
Abstract Cutinases (EC 3.1. 1.74) belong to the α/β-hydrolase superfamily. They were
initially discovered because they are secreted by fungi to hydrolyze the ester bonds of the …

[HTML][HTML] Towards bio-upcycling of polyethylene terephthalate

T Tiso, T Narancic, R Wei, E Pollet, N Beagan… - Metabolic …, 2021 - Elsevier
Over 359 million tons of plastics were produced worldwide in 2018, with significant growth
expected in the near future, resulting in the global challenge of end-of-life management. The …

Structural insights into (tere) phthalate-ester hydrolysis by a carboxylesterase and its role in promoting PET depolymerization

G von Haugwitz, X Han, L Pfaff, Q Li, H Wei, J Gao… - ACS …, 2022 - ACS Publications
TfCa, a promiscuous carboxylesterase from Thermobifida fusca, was found to hydrolyze
polyethylene terephthalate (PET) degradation intermediates such as bis (2-hydroxyethyl) …

Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate

R Wei, W Zimmermann - Microbial biotechnology, 2017 - Wiley Online Library
The global production of fossil-based plastics has grown more than 20-fold since 1964 to
322 million tons in 2015, and a slowdown of this rate is not expected (Ellen MacArthur …

Engineering and evaluation of thermostable IsPETase variants for PET degradation

S Brott, L Pfaff, J Schuricht, JN Schwarz… - Engineering in life …, 2022 - Wiley Online Library
Polyethylene terephthalate (PET) is a mass‐produced petroleum‐based synthetic polymer.
Enzymatic PET degradation using, for example, Ideonella sakaiensis PETase (IsPETase) …

Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis

B Liu, L He, L Wang, T Li, C Li, H Liu, Y Luo… - …, 2018 - Wiley Online Library
Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing
of poly (ethylene terephthalate)(PET) waste. PETase, a newly identified enzyme from …

Enzymatic surface hydrolysis of PET: effect of structural diversity on kinetic properties of cutinases from Thermobifida

E Herrero Acero, D Ribitsch, G Steinkellner… - …, 2011 - ACS Publications
In this study cutinases from Thermobifida cellulosilytica DSM44535 (Thc_Cut1 and
Thc_Cut2) and Thermobifida fusca DSM44342 (Thf42_Cut1) hydrolyzing poly (ethylene …

Current progress on the biodegradation of synthetic plastics: from fundamentals to biotechnological applications

R Andler, T Tiso, L Blank, C Andreeßen… - … Science and Bio …, 2022 - Springer
Plastic pollution is a global concern due to the long half-life and high resistance of many
synthetic plastics to natural biodegradation. Therefore, great effort is required to avoid …

Structural and functional studies on a thermostable polyethylene terephthalate degrading hydrolase from Thermobifida fusca

C Roth, R Wei, T Oeser, J Then, C Föllner… - Applied microbiology …, 2014 - Springer
Bacterial cutinases are promising catalysts for the modification and degradation of the
widely used plastic polyethylene terephthalate (PET). The improvement of the enzyme for …