A critical review of microfluidic systems for CRISPR assays

AS Avaro, JG Santiago - Lab on a Chip, 2023 - pubs.rsc.org
Reviewed are nucleic acid detection assays that incorporate clustered regularly interspaced
short palindromic repeats (CRISPR)-based diagnostics and microfluidic devices and …

Advances and challenges in synthetic biology for mosquito control

SC Weng, RA Masri, OS Akbari - Trends in parasitology, 2024 - cell.com
Mosquito-borne illnesses represent a significant global health peril, resulting in
approximately one million fatalities annually. West Nile, dengue, Zika, and malaria are …

Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2

A Mahas, T Marsic… - Proceedings of the …, 2022 - National Acad Sciences
Type VI CRISPR-Cas systems have been repurposed for various applications such as gene
knockdown, viral interference, and diagnostics. However, the identification and …

New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity

AM Molina Vargas, S Sinha, R Osborn… - Nucleic acids …, 2024 - academic.oup.com
An increasingly pressing need for clinical diagnostics has required the development of novel
nucleic acid-based detection technologies that are sensitive, fast, and inexpensive, and that …

Rapid detection of SARS-CoV-2 RNA in saliva via Cas13

SS Chandrasekaran, S Agrawal, A Fanton… - Nature biomedical …, 2022 - nature.com
Rapid nucleic acid testing is central to infectious disease surveillance. Here, we report an
assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device …

Targeting sex determination to suppress mosquito populations

M Li, NP Kandul, R Sun, T Yang, ED Benetta… - Elife, 2024 - elifesciences.org
Each year, hundreds of millions of people are infected with arboviruses such as dengue,
yellow fever, chikungunya, and Zika, which are all primarily spread by the notorious …

A post-pandemic perspective: Evolution of SARS-CoV-2 early detection

Y Zhu, W Jiang, R Chen, J Jouha, Q Wang, L Wu… - TrAC Trends in …, 2024 - Elsevier
The COVID-19 pandemic, caused by the rapid spread of the coronavirus 2 (SARS-CoV-2),
has resulted in significant global loss of life and property. While reverse transcription …

CRISPR–Cas12a-Mediated Hue-Recognition Lateral Flow Assay for Point-of-Need Detection of Salmonella

J Yuan, L Wang, L Huang, K He, H Wang… - Analytical …, 2023 - ACS Publications
Nucleic acid detection of pathogens in a point-of-need (PON) manner is of great significance
yet remains challenging for sensitive and accurate visual discrimination. Here, we report a …

CRISPR/Cas and Argonaute-powered lateral flow assay for pathogens detection

Y Li, L Zhao, L Ma, Y Bai, F Feng - Critical Reviews in Food …, 2024 - Taylor & Francis
Pathogens contamination is a pressing global public issue that has garnered significant
attention worldwide, especially in light of recent outbreaks of foodborne illnesses …

Engineered antiviral sensor targets infected mosquitoes

E Dalla Benetta, AJ López-Denman, HH Li… - The CRISPR …, 2023 - liebertpub.com
Escalating vector disease burdens pose significant global health risks, as such innovative
tools for targeting mosquitoes are critical. CRISPR-Cas technologies have played a crucial …