Insights into the assembly and regulation of the bacterial divisome

TA Cameron, W Margolin - Nature Reviews Microbiology, 2024 - nature.com
The ability to split one cell into two is fundamental to all life, and many bacteria can
accomplish this feat several times per hour with high accuracy. Most bacteria call on an …

Splitsville: structural and functional insights into the dynamic bacterial Z ring

DP Haeusser, W Margolin - Nature Reviews Microbiology, 2016 - nature.com
Bacteria must divide to increase in number and colonize their niche. Binary fission is the
most widespread means of bacterial cell division, but even this relatively simple mechanism …

A method for detergent-free isolation of membrane proteins in their local lipid environment

SC Lee, TJ Knowles, VLG Postis, M Jamshad… - Nature protocols, 2016 - nature.com
Despite the great importance of membrane proteins, structural and functional studies of
these proteins present major challenges. A significant hurdle is the extraction of the …

Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell division

P Szwedziak, Q Wang, TAM Bharat, M Tsim, J Löwe - Elife, 2014 - elifesciences.org
Membrane constriction is a prerequisite for cell division. The most common membrane
constriction system in prokaryotes is based on the tubulin homologue FtsZ, whose filaments …

Membrane mimetic chemistry in artificial cells

JA Vance, NK Devaraj - Journal of the American Chemical Society, 2021 - ACS Publications
Lipid membranes in cells are fluid structures that undergo constant synthesis, remodeling,
fission, and fusion. The dynamic nature of lipid membranes enables their use as adaptive …

De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns

E Godino, JN López, D Foschepoth, C Cleij… - Nature …, 2019 - nature.com
The Min biochemical network regulates bacterial cell division and is a prototypical example
of self-organizing molecular systems. Cell-free assays relying on purified proteins have …

The keepers of the ring: regulators of FtsZ assembly

C Ortiz, P Natale, L Cueto… - FEMS microbiology …, 2016 - academic.oup.com
FtsZ, a GTPase distributed in the cytoplasm of most bacteria, is the major component of the
machinery responsible for division (the divisome) in Escherichia coli. It interacts with …

Treadmilling analysis reveals new insights into dynamic FtsZ ring architecture

DA Ramirez-Diaz, DA García-Soriano, A Raso… - PLoS …, 2018 - journals.plos.org
FtsZ, the primary protein of the bacterial Z ring guiding cell division, has been recently
shown to engage in intriguing treadmilling dynamics along the circumference of the division …

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

ACK Teo, SC Lee, NL Pollock, Z Stroud, S Hall… - Scientific Reports, 2019 - nature.com
Biological characterisation of membrane proteins lags behind that of soluble proteins. This
reflects issues with the traditional use of detergents for extraction, as the surrounding lipids …

Cell age dependent concentration of Escherichia coli divisome proteins analyzed with ImageJ and ObjectJ

NOE Vischer, J Verheul, M Postma… - Frontiers in …, 2015 - frontiersin.org
The rod-shaped Gram-negative bacterium Escherichia coli multiplies by elongation followed
by binary fission. Longitudinal growth of the cell envelope and synthesis of the new poles …