Ribonucleases (RNases) are mediators in most reactions of RNA metabolism. In recent
years, there has been a surge of new information about RNases and the roles they play in …

Adaptation to fluctuations in nutrient availability is a fact of life for single-celled organisms in
the'wild'. A decade ago our understanding of how bacteria adjust cell cycle parameters to …

Genome‐wide screens have discovered a large set of essential genes in the opportunistic
human pathogen Streptococcus pneumoniae. However, the functions of many essential …

The continuous degradation and synthesis of prokaryotic mRNAs not only give rise to the
metabolic changes that are required as cells grow and divide but also rapid adaptation to …

Comparative genomics is the cornerstone of identification of gene functions. The immense
number of living organisms precludes experimental identification of functions except in a …

Like eukaryotes, bacteria must coordinate division with growth to ensure cells are the
appropriate size for a given environmental condition or developmental fate. As single-celled …

Protein–protein interactions are fundamental to all biological processes, and a
comprehensive determination of all protein–protein interactions that can take place in an …

In all organisms, multi-subunit replicases are responsible for the accurate duplication of
genetic material during cellular division. Initiator proteins control the onset of DNA …

Chromosomal replication must be limited to once and only once per cell cycle. This is
accomplished by multiple regulatory pathways that govern initiator proteins and replication …

The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …