Charged and polar groups, through forming ion pairs, hydrogen bonds, and other less
specific electrostatic interactions, impart important properties to proteins. Modulation of the …

Understanding the function of biological macromolecules and their complexes at the
physicochemical level requires knowledge of both their structure and dynamics …

The structure of a protein complex together with information about its affinity and other
thermodynamic characteristics provide a “frozen” view of the complex. This picture ignores …

The formation of protein complexes involves an encounter complex, in which proteins show
few specific interactions and assume many orientations. Recent kinetic and structural …

Molecules containing unpaired electrons affect the magnetic properties of nuclei in their
vicinity, which is observable in Nuclear Magnetic Resonance (NMR) spectra. As these …

Cytochrome P450cam catalyzes the hydroxylation of camphor in a complex process
involving two electron transfers (ETs) from the iron-sulfur protein putidaredoxin. The …

Microsomal cytochrome b 5 (cytb 5) is a membrane-bound protein that modulates the
catalytic activity of its redox partner, cytochrome P4502B4 (cytP450). Here, we report the first …

A striking feature of nucleic acids and lipid membranes is that they all carry net negative
charge and so is true for the majority of intracellular proteins. It is suggested that the role of …

Calmodulin (CaM) is the universal calcium sensor in eukaryotes, regulating the function of
numerous proteins. Crystallography and NMR show that free CaM-4Ca2+ exists in an …

Recent studies have provided experimental evidence for the existence of an encounter
complex, a transient intermediate in the formation of protein complexes. We use …